Abstract
Despite their relatively lower efficiency, nonviral approaches are emerging as safer alternatives in gene therapy to viral vectors. Delivery of nucleic acids to the target site is an important factor for effective gene expression (plasmid DNA) or knockdown (siRNA) with minimal side effects. Direct deposition at the target site by physical methods, including ultrasound, electroporation and gene gun, is one approach for local delivery. For less accessible sites, the development of carriers that can home into the target tissue is required. Cationic peptides, lipoplexes, polyplexes and nanoplexes have been used as carriers for delivery of nucleic acids. Targeting ligands, such as cell targeting peptides, have also been applied to decorate delivery vehicles in order to enhance their efficacy. This review focuses on delivery strategies and recent progress in non-viral carriers and their modifications to improve their performance in targeting and transfection.
Keywords: Gene delivery, lipoplexes, nanoplexes, nonviral, polyplexes, peptides, targeting, (plasmid DNA), Cationic peptides, nucleic acids (NAs)
Current Medicinal Chemistry
Title: Nonviral Approach for Targeted Nucleic Acid Delivery
Volume: 19 Issue: 2
Author(s): M. Jafari, M. Soltani, S. Naahidi, D. N. Karunaratne and P. Chen
Affiliation:
Keywords: Gene delivery, lipoplexes, nanoplexes, nonviral, polyplexes, peptides, targeting, (plasmid DNA), Cationic peptides, nucleic acids (NAs)
Abstract: Despite their relatively lower efficiency, nonviral approaches are emerging as safer alternatives in gene therapy to viral vectors. Delivery of nucleic acids to the target site is an important factor for effective gene expression (plasmid DNA) or knockdown (siRNA) with minimal side effects. Direct deposition at the target site by physical methods, including ultrasound, electroporation and gene gun, is one approach for local delivery. For less accessible sites, the development of carriers that can home into the target tissue is required. Cationic peptides, lipoplexes, polyplexes and nanoplexes have been used as carriers for delivery of nucleic acids. Targeting ligands, such as cell targeting peptides, have also been applied to decorate delivery vehicles in order to enhance their efficacy. This review focuses on delivery strategies and recent progress in non-viral carriers and their modifications to improve their performance in targeting and transfection.
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Cite this article as:
Jafari M., Soltani M., Naahidi S., N. Karunaratne D. and Chen P., Nonviral Approach for Targeted Nucleic Acid Delivery, Current Medicinal Chemistry 2012; 19 (2) . https://dx.doi.org/10.2174/092986712803414141
DOI https://dx.doi.org/10.2174/092986712803414141 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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Chalcogen-modified nucleic acid analogues
Chalcogen-modified nucleosides, nucleotides and oligonucleotides have been of great interest to scientific research for many years. The replacement of oxygen in the nucleobase, sugar or phosphate backbone by chalcogen atoms (sulfur, selenium, tellurium) gives these biomolecules unique properties resulting from their altered physical and chemical properties. The continuing interest in ...read more
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