Title:Method Development and Validation of Stability-Indicating HPLC-UV Method for Determination of 5 -Fluorouracil
Volume: 10
Author(s): Diksha Gulati, Deepshi Arora*, Yugam Taneja and Ashwani Dhingra*
Affiliation:
- Guru Gobind Singh College of Pharmacy, Yamunanagar, Haryana, 135001, India
- Guru Gobind Singh College of Pharmacy, Yamunanagar, Haryana, 135001, India
Keywords:
Fluorouracil, validation, linearity, quantification, robustness, accuracy.
Abstract:
Introduction: In the realm of pharmaceutical analysis, the development and validation
of a High-Performance Liquid Chromatography with Ultraviolet Detection (HPLC-UV) method
for the precise determination of 5-FU stand as a crucial endeavor.
Methods: This study outlines the systematic process of method development, optimization, and
subsequent validation to ensure the reliability and applicability of the method in pharmaceutical
quality control. Method optimization included the selection of an appropriate stationary phase,
mobile phase composition, and chromatographic conditions to achieve optimum resolution, sensitivity,
and run time.
Results: A speedy, simple, and dependable HPLC technique for quantifying 5-FU on a C18 (250
4.6 mm, 5 m particle size) column with solvent A included 10 mM phosphate buffer with pH 3.0
and solvent B with acetonitrile as the mobile phase. At 250 nm, the effluent was measured. Further,
5-FU had a retention time of 1.698 minutes.
Conclusion: According to ICH criteria, the devised technique was validated for precision, accuracy,
linearity range, robustness, and ruggedness.
