Title:The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
Volume: 20
Issue: 2
Author(s): Willem Delabie, Dominique De Bleser, Vicky Vandewalle, Marie-Laurence De Prest, Philippe Vandekerckhove, Veerle Compernolle and Hendrik B. Feys*
Affiliation:
- Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium
- Department
of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
Keywords:
Human platelet lysate, pathogen inactivation, mesenchymal stem cells, amotosalen, mass spectrometry, stem cells.
Abstract:
Background: Human Platelet Lysate (hPL) is a platelet-derived and growth factor-rich
supplement for cell culture. It can be prepared from surplus platelet concentrates initially intended
for transfusion. Amotosalen-based photochemical pathogen inactivation of platelet concentrates is
used in a number of blood establishments worldwide to minimize the risk of pathogen transmission
from donor to patient.
Method: This pathogen inactivation method has not been formally validated for direct use on
hPL. Here, we have studied the impact of pathogen inactivation on hPL and compared it to untreated
hPL prepared from pathogen-inactivated platelet concentrates or control hPL. We used mass
spectrometry, ELISA, and in vitro mesenchymal stem cell culture for determining residual amotosalen,
final growth factor content, and cell doubling, respectively.
Result: The data have shown amotosalen concentrations to be reduced a thousand-fold following
pathogen inactivation, leaving trace quantities of photosensitizer molecules in the final hPL product.
Some growth factors have been reported to be significantly more impacted in hPL that is directly
pathogen-inactivated compared to both control conditions. This has no significant effect on
the growth kinetics of adipose-derived mesenchymal stem cells.
Conclusion: Direct amotosalen-based pathogen inactivation has a measurable impact on certain
growth factors in hPL, but this does not outweigh the likely benefits of reducing the odds of
donor-to-patient pathogen transmission.
