Title:Biogenic Silver Nanoparticles for the Estimation of Lead in Chocolates
Volume: 21
Issue: 2
Author(s): Sabarivasan Mohan*Stalin Selvaraj*
Affiliation:
- Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology, SASTRA
Deemed to be University Thanjavur, 613 401, India
- Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology, SASTRA
Deemed to be University Thanjavur, 613 401, India
Keywords:
Biogenic silver nanoparticles, colorimetric sensor, lead, dark chocolates, limit of detection (LOD), limit of quantification (LOQ).
Abstract:
Background: This study aimed to demonstrate the practical and sensitive lead detection
using biogenic silver nanoparticles in dark chocolate samples and silver nitrate was reduced using
Syzygium cumini aqueous seed extract as a reducing agent to produce nanoparticles. The obtained
NPs were characterized via spectrometry and scanning electron microscopy.
Method: Silver nitrate was reduced using Syzygium cumini aqueous seed extract as a reducing agent to
produce nanoparticles. The obtained NPs were characterized via spectrometry and scanning electron
microscopy. The formation of NPs was confirmed by a shift in lambda max to 430 nm and the observation
of 2 nm-sized particles in SEM. Data on change in lambda max as a function of time must be
shown in a figure. Silver nanoparticles mixed with lead induce a red shift from the original 430 nm to
434 nm absorption maximum, confirming the efficiency of silver nanoparticles in finding a lead.
Result: We evaluated that the limit of detection (LOD) was to be 0.8715 µg/mL, and the limit of
quantification (LOQ) was found to be 150 µg/ mL.
Conclusion: The prepared biogenic silver nanoparticles have been used to detect lead in spiked diluted
chocolates.
