Serum MicroRNAs as Predictors of Diagnosis and Drug-resistance in Temporal Lobe Epilepsy: A Preliminary Study

Title:Serum MicroRNAs as Predictors of Diagnosis and Drug-resistance in Temporal Lobe Epilepsy: A Preliminary Study

Volume: 22 Issue: 14

Author(s): Gloria Bertoli*, Francesco Fortunato, Claudia Cava, Ida Manna*, Francesca Gallivanone, Angelo Labate, Antonella Panio, Danilo Porro and Antonio Gambardella*

Affiliation:

• Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, Segrate, Milan, Italy
• NBFC, National Biodiversity Future Center, Palermo 90133, Italy

• IBFM-CNR, Section of Germaneto, Catanzaro, Italy

• Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, Segrate, Milan, Italy
• Institute of Neurology, Department of Medical and Surgical Sciences, University 'Magna Graecia', Germaneto, Catanzaro, Italy;

Keywords: Temporal lobe epilepsy, TLE, microRNAs, miRNAs, diagnosis, prediction of therapy response, prognosis, circulating biomarkers.

Abstract: Objective: Temporal lobe epilepsy (TLE) is the most common form of refractory focal epilepsy, and the current clinical diagnosis is based on EEG, clinical neurological history and neuroimaging findings.

Methods: So far, there are no blood-based molecular biomarkers of TLE to support clinical diagnosis, despite the pathogenic mechanisms underlying TLE involving defects in the regulation of gene expression. MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression.

Results: Recent studies show the feasibility of detecting miRNAs in body fluids; circulating miRNAs have emerged as potential clinical biomarkers in epilepsy, although the TLE miRNA profile needs to be addressed. Here, we analysed the diagnostic potential of 8 circulating miRNAs in sera of 52 TLE patients and 40 age- and sex-matched donor controls by RT-qPCR analyses.

Conclusion: We found that miR-34a-5p, -106b-5p, -130a-3p, -146a-5p, and -19a-3p are differently expressed in TLE compared to control subjects, suggesting a diagnostic role. Furthermore, we found that miR-34a-5p, -106b-5p, -146a-5p and miR-451a could become prognostic biomarkers, being differentially expressed between drug-resistant and drug-responsive TLE subjects. Therefore, serum miRNAs are diagnostic and drug-resistance predictive molecules of TLE.

Cite this article as:

Bertoli Gloria*, Fortunato Francesco, Cava Claudia, Manna Ida*, Gallivanone Francesca, Labate Angelo, Panio Antonella, Porro Danilo and Gambardella Antonio*, Serum MicroRNAs as Predictors of Diagnosis and Drug-resistance in Temporal Lobe Epilepsy: A Preliminary Study, Current Neuropharmacology 2024; 22 (14) . https://dx.doi.org/10.2174/1570159X22666240516145823

DOI https://dx.doi.org/10.2174/1570159X22666240516145823	Print ISSN 1570-159X
Publisher Name Bentham Science Publisher	Online ISSN 1875-6190