Title:Optimizing Neuroprotective Nano-structured Lipid Carriers for Transdermal Delivery through Artificial Neural Network
Volume: 13
Issue: 1
Author(s): Saloni Dalwadi, Vaishali Thakkar*Bhupendra Prajapati
Affiliation:
- Department of Pharmaceutics, Anand
Pharmacy College, Anand, Gujarat, 388001, India
Keywords:
Artificial neural network, design of experiment, donepezil HCl, memantine HCl, nano-structured lipid carrier, box behnken design.
Abstract:
Background: Dementia associated with Alzheimer’s disease (AD) is a neurological disorder.
AD is a progressive neurodegenerative condition that predominantly impacts the elderly population,
although it can also manifest in younger people through the impairment of cognitive functions,
such as memory, cognition, and behaviour. Donepezil HCl and Memantine HCl are encapsulated
in Nanostructured Lipid Carriers (NLCs) to prolong systemic circulation and minimize the systemic
side effects.
Objective: This work explores the use of data mining tools to optimize the formulation of NLCs
comprising of Donepezil HCl and Memantine HCl for transdermal drug delivery. Neuroprotective
drugs and excipients are utilized for protecting the nervous system against damage or degeneration.
Methods: The NLCs were formulated using a high-speed homogenization technique followed by ultrasonication.
NLCs were optimized using Box Behnken Design (BBD) in Design Expert Software
and artificial neural network (ANN) in IBM SPSS statistics. The independent variables included the
ratio of solid lipid to liquid lipid, the percentage of surfactant, and the revolutions per minute (RPM)
of the high-speed homogenizer.
Results: The NLCs that were formulated had a mean particle size ranging from 67.0±0.45 to
142.4±0.52 nm. Both drugs have a %EE range over 75%, and Zeta potential was determined to be -
26±0.36 mV. CryoSEM was used to do the structural study. The permeation study showed the prolonged
release of the formulation.
Conclusion: The results indicate that NLCs have the potential to be a carrier for transporting medications
to deeper layers of the skin and reaching systemic circulation, making them a suitable formulation
for the management of Dementia. Both ANN and BBD techniques are effective tools for systematically
developing and optimizing NLC formulation.
