Title:Knockdown of Ubiquitin-Conjugating Enzyme E2 T Abolishes the
Progression of Head and Neck Squamous Cell Carcinoma by Inhibiting
NF-Κb Signaling and inducing Ferroptosis
Volume: 25
Issue: 7
Author(s): Feng Cai, Hongbo Xu, Shilong Song, Gengming Wang, Yajun Zhang, Jing Qian and Lu Xu*
Affiliation:
- Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Zhihuai Road, Bengbu,
233000, Anhui, China
Keywords:
UBE2T, NF-κB, ferroptosis, HNSC, tumor, squamous cell carcinoma.
Abstract:
Background: Ubiquitin-conjugating enzyme 2T (UBE2T) has been reported to be associated
with uncontrolled cell growth and tumorigenesis in multiple cancer types. However, the understanding
of its regulatory role in the carcinogenesis of Head And Neck Squamous Cell Carcinoma
(HNSC) is limited.
Methods: UBE2T expression in HNSC patient samples and the correlation between its expression
and patients’ survival rates were evaluated using The Cancer Genome Atlas (TCGA) database.
Cell survival and proliferation were investigated in UM-SCC1 and UM-SCC15 cells infected with
control and shUBE2T lentivirus. The xenograft mouse model was established using UM-SCC15
cells to examine HNSC tumorigenesis with or without UBE2T. Western blot, qRT-PCR, and ferroptosis
assays were carried out to disclose the interaction between UBE2T and NF-κB signaling
and ferroptosis.
Results: The increased expression of UBE2T was noted in tumor tissues of patients with HNSC,
correlating with a significantly reduced overall survival time in this patient cohort. Knockdown of
UBE2T inhibited HNSC tumorigenesis and tumor growth. Mechanistically, inhibition of UBE2T
suppressed NF-κB signaling and induced ferroptosis in HNSC.
Conclusion: Our study underscores the multifaceted role of UBE2T in HNSC, illuminating its potential
as a biomarker and therapeutic target.
