![conference banner](/images/conference-banner.jpeg)
摘要
肥胖是一个日益严重的全球健康问题,导致各种慢性疾病。尽管有标准的治疗方案,但肥胖的患病率仍在持续上升,这强调了对新药的需求。体外药物发现研究方法为鉴定新的抗肥胖药物提供了一个节省时间和成本的平台。该综述涵盖了使用体外模型的肥胖和药物发现研究的各个方面。除了讨论病因、诊断、预防和治疗外,本文还重点介绍了体外研究的优点和局限性,并详尽地涵盖了基于不同动物和人类的酶和细胞系的模型。与传统的体内动物研究相比,使用酶和细胞系为基础的体外临床前试验为抗肥胖药物的开发提供了几个优势。这些方法快速,经济实惠,并提供高通量筛选。它们还可以提供有关药物靶标相互作用、作用方式和毒性概况的深刻信息。通过揭示导致肥胖的因素,体外测试也为个性化治疗提供了机会。技术将继续发展,导致更精确、更可靠的体外检测的产生,这将在寻找新型抗肥胖药物方面变得越来越重要。
关键词: 胰脂肪酶抑制,肥胖和超重,BMI,细胞系,临床前试验,体重管理,抗肥胖药物。
[http://dx.doi.org/10.4239/wjd.v1.i3.76] [PMID: 21537431]
[PMID: 21640051]
[http://dx.doi.org/10.1111/j.1467-789X.2009.00712.x] [PMID: 20122135]
[http://dx.doi.org/10.1111/j.1467-789X.2006.00230.x] [PMID: 16866975]
[http://dx.doi.org/10.1016/j.cell.2013.12.012] [PMID: 24439368]
[http://dx.doi.org/10.1016/j.cmet.2018.11.002] [PMID: 30503034]
[http://dx.doi.org/10.1002/mnfr.201900995] [PMID: 31786828]
[http://dx.doi.org/10.1002/mnfr.201600746] [PMID: 27873458]
[http://dx.doi.org/10.1038/ncomms6493] [PMID: 25423280]
[http://dx.doi.org/10.2174/092986709787315568] [PMID: 19199918]
[http://dx.doi.org/10.1016/j.orcp.2015.12.004] [PMID: 26740473]
[http://dx.doi.org/10.2174/2215083803666170309124540]
[http://dx.doi.org/10.1016/j.amjmed.2016.02.009]
[http://dx.doi.org/10.53879/id.53.07.10479]
[http://dx.doi.org/10.17305/bjbms.2019.4378] [PMID: 31621554]
[http://dx.doi.org/10.1016/j.biomaterials.2018.10.030] [PMID: 30384974]
[http://dx.doi.org/10.3390/ijms24043652] [PMID: 36835060]
[http://dx.doi.org/10.1074/jbc.M502123200] [PMID: 16179352]
[http://dx.doi.org/10.1080/17460441.2023.2156499] [PMID: 36508256]
[http://dx.doi.org/10.1111/j.1574-6968.2007.00917.x] [PMID: 17937667]
[http://dx.doi.org/10.3389/fnut.2022.844195] [PMID: 35284458]
[http://dx.doi.org/10.3389/fphar.2018.00756] [PMID: 30050443]
[http://dx.doi.org/10.3390/foods11131964] [PMID: 35804780]
[http://dx.doi.org/10.3390/molecules27165159] [PMID: 36014395]
[http://dx.doi.org/10.1016/j.heliyon.2020.e04392] [PMID: 32671273]
[http://dx.doi.org/10.1016/B978-0-12-803077-6.00009-6]
[http://dx.doi.org/10.1016/0092-8674(74)90116-0] [PMID: 4426090]
[http://dx.doi.org/10.1038/s41598-023-30227-5] [PMID: 36806746]
[http://dx.doi.org/10.1186/s12986-023-00732-5] [PMID: 36774476]
[http://dx.doi.org/10.1016/j.fshw.2022.10.043]
[http://dx.doi.org/10.1152/physrev.1998.78.3.783] [PMID: 9674695]
[http://dx.doi.org/10.1016/j.diff.2020.08.003] [PMID: 32891960]
[http://dx.doi.org/10.1016/j.bbagen.2015.09.014] [PMID: 26391843]
[http://dx.doi.org/10.1016/j.bbrc.2022.11.042] [PMID: 36442234]
[http://dx.doi.org/10.1186/s12906-020-2824-x] [PMID: 32024512]
[http://dx.doi.org/10.1371/journal.pone.0218792] [PMID: 31226166]
[http://dx.doi.org/10.1194/jlr.D500037-JLR200] [PMID: 16319419]
[http://dx.doi.org/10.1371/journal.pone.0112123] [PMID: 25409310]
[http://dx.doi.org/10.1126/science.8066449] [PMID: 8066449]
[http://dx.doi.org/10.1038/s41598-023-27424-7] [PMID: 36609640]
[http://dx.doi.org/10.1007/s13105-022-00876-7] [PMID: 35122619]
[http://dx.doi.org/10.1016/j.tiv.2020.104904] [PMID: 32473317]
[PMID: 4357355]
[http://dx.doi.org/10.1152/ajpcell.00519.2020] [PMID: 33439778]
[http://dx.doi.org/10.1089/scd.2014.0130] [PMID: 25428599]
[http://dx.doi.org/10.1016/j.phymed.2013.10.009] [PMID: 24252344]
[http://dx.doi.org/10.1002/jcb.25204] [PMID: 25903991]
[http://dx.doi.org/10.1097/JCMA.0000000000000655] [PMID: 34966163]
[http://dx.doi.org/10.1016/j.bbrc.2011.05.042] [PMID: 21605549]
[http://dx.doi.org/10.3892/mmr.2017.7450] [PMID: 28901521]
[http://dx.doi.org/10.1007/978-1-59745-019-5_2]
[http://dx.doi.org/10.1038/ncomms7792] [PMID: 25881961]
[http://dx.doi.org/10.1007/s00125-012-2809-5] [PMID: 23314846]
[http://dx.doi.org/10.1007/s11626-021-00617-y] [PMID: 34811702]
[http://dx.doi.org/10.1074/jbc.M111.248435] [PMID: 21669876]
[http://dx.doi.org/10.1242/jcs.111.22.3311] [PMID: 9788873]
[http://dx.doi.org/10.1371/journal.pone.0071576] [PMID: 23951196]
[http://dx.doi.org/10.3390/ijms17071040] [PMID: 27376273]
[http://dx.doi.org/10.2174/156652411795677990] [PMID: 21506921]
[http://dx.doi.org/10.1139/bcb-2014-0011] [PMID: 24798646]
[http://dx.doi.org/10.1055/s-0033-1334896] [PMID: 23468252]
[http://dx.doi.org/10.1111/jpn.12392] [PMID: 26452529]
[http://dx.doi.org/10.1016/j.mce.2009.01.019] [PMID: 19418627]
[http://dx.doi.org/10.1016/j.endonu.2013.03.011] [PMID: 23834768]
[http://dx.doi.org/10.4252/wjsc.v6.i3.312] [PMID: 25126381]
[http://dx.doi.org/10.1111/ijfs.16269]
[http://dx.doi.org/10.1016/j.mce.2022.111555] [PMID: 35031432]
[http://dx.doi.org/10.1371/journal.pone.0113620] [PMID: 25419971]
[http://dx.doi.org/10.3389/fphar.2021.707507] [PMID: 34483915]
[http://dx.doi.org/10.4161/adip.29385] [PMID: 25068086]
[http://dx.doi.org/10.1016/j.steroids.2006.04.008] [PMID: 16762384]
[http://dx.doi.org/10.1002/oby.21896] [PMID: 28649807]
[http://dx.doi.org/10.1210/en.2005-0989] [PMID: 16195405]
[http://dx.doi.org/10.1016/S0091-679X(08)61571-X] [PMID: 9648099]
[http://dx.doi.org/10.1007/s11626-017-0203-9] [PMID: 29197027]
[http://dx.doi.org/10.1038/bjc.2014.166] [PMID: 25117809]
![Wayfinder Image](/images/wayfinder.jpg)