Title:Exploring the Mechanism of Bufei Decoction in the Treatment of Bronchial Asthma Based on Network Pharmacology and Molecular Docking
Volume: 28
Issue: 5
Author(s): Yong-Guang Han, Xing Lv, Ya-Lan Tan, Yun-Shan Ding, Chao-Yun Zhang and Hua Bian*
Affiliation:
- Henan Key Laboratory of Zhang Zhong-jing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang 473004, China
Keywords:
PI3K-Akt signaling pathway, bufei decoction, bronchial asthma, network pharmacology, molecular docking, pharmacological mechanism.
Abstract:
Background: Bufei decoction (BFD) is used in clinical practice to treat bronchial
asthma (BA), although its molecular mechanism of action remains unclear.
Objective: This study aimed to explore the molecular mechanism of BFD for treating BA.
Methods: Network pharmacology and molecular docking predicted the molecular mechanism
and the analysis results were verified using the ELISA kit and RT-qPCR.
Results: There were 58 main active components and 121 potential targets in the BFD from the
Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(
TCMSP), and 11 core targets were obtained from the protein-protein interactions(PPI)
network. The gene ontology (GO) analysis found that the treatment of BA with BFD was mainly
related to inflammatory reaction, membrane raft, cytokine activity, etc. The Kyoto Encyclopedia
of Genes and Genomes (KEGG) pathway enrichment analysis showed that it was mainly related
to interleukin (IL)-17 signaling pathway, tumor necrosis factor (TNF) signaling pathway, PI3KAkt
signaling pathway, etc. The molecular docking results showed that the main active ingredients
had strong binding ability with core targets. BFD significantly reduced the TNF-α, IL-6,
and IL-1β and increased the level of IL-10 in rats with BA. BFD also significantly reduced the
mRNA level of PI3K, AKT1, and VEGFA while increasing the mRNA level of TP53 in rats.
Conclusion: This study used network pharmacology methods to predict the potential active ingredients,
targets, and pathways of BFD in treating BA and explore its possible molecular
mechanism, which provided a theoretical basis for further study.
