Title:Analgesic and Anti-inflammatory Potential of the New Tetrahydropyran
Derivative (2s,6s)-6-ethyl-tetrahydro-2h-pyran-2-yl) Methanol
Volume: 23
Issue: 2
Author(s): Gustavo Nunes de Santana Castro, Raquel do Nascimento de Souza, Alba Cenélia Matos da Silva, Roberto Laureano-Melo, Wellington da Silva Côrtes, Saulo Luis Capim, Mário Luiz Araujo de Almeida Vasconcellos and Bruno Guimarães Marinho*
Affiliation:
- Laboratório de Farmacologia da Inflamação e Nocicepção, Programa de Pós-Graduação em Medicina Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brasil
- Laboratório de Cultura de Células, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brasil
- Laboratório de Psicofarmacologia e Comportamento, Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brasil
Keywords:
Tetrahydropyran derivative, mice, antinociceptive effect, opioid system, cytokines, inflammation.
Abstract:
Background: The development of analgesic and anti-inflammatory drugs plays a crucial
role in modern medicine, aiming to alleviate pain and reduce inflammation in patients. Opioids and
nonsteroidal anti-inflammatory drugs are groups of drugs conventionally used to treat pain and inflammation,
but a wide range of adverse effects and ineffectiveness in some pathological conditions
leads us to search for new drugs with analgesic and anti-inflammatory properties.
Objectives: In this regard, the authors intend to investigate the ((2s,6s)-6-ethyl-tetrahydro-2h-pyran-
2-yl) methanol compound (LS20) on pain and acute inflammation.
Methods: Male Swiss mice were evaluated using acetic acid-induced abdominal writhing, formalin,
and tail-flick as models of nociceptive evaluation and edema paw, air pouch and cell culture as
models of inflammatory evaluation besides the rotarod test for assessment of motor impairment.
Results: The compound showed an effect on the acetic acid-induced abdominal writhing, formalin
and tail-flick tests. Studying the mechanism of action, reversion of the antinociceptive effect of the
compound was observed from previous intraperitoneal administration of selective and non-selective
opioid antagonists on the tail flick test. In addition, the compound induced an antiedematogenic
effect and reduced leukocyte migration and the production of pro-inflammatory cytokines in the air
pouch model. LS20 was able to maintain cell viability, in addition to reducing cell production of
TNF-α and IL-6.
Conclusion: In summary, the LS20 compound presented an antinociceptive effect, demonstrating
the participation of the opioid system and an anti-inflammatory effect related to the inhibition of
pro-inflammatory cytokine production. The compound also demonstrated safety at the cellular level.
