Title:Cinnamic Acid Ameliorates Acetic Acid-induced Inflammatory Response
through Inhibition of TLR-4 in Colitis Rat Model
Volume: 23
Issue: 1
Author(s): Zahra Rezaei, Saeideh Momtaz*, Pardis Gharazi, Mahban Rahimifard, Maryam Baeeri, Ali Reza Abdollahi, Mohammad Abdollahi, Amirhossein Niknejad, Danial Khayatan, Mohammad Hosein Farzaei and Amir Hossein Abdolghaffari*
Affiliation:
- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
- Department of Toxicology and Pharmacology, Faculty of Pharmacy, And Toxicology & Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences, Tehran, 1417614411, Iran
- Gastrointestinal Pharmacology Interest Group (GPIG), Universal Scientific Education and Research Network (USERN), Tehran, Iran
- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
- Gastrointestinal Pharmacology Interest Group (GPIG), Universal Scientific Education and Research Network (USERN), Tehran, Iran
Keywords:
Cinnamic acid, Inflammatory bowel disease (IBD), Interleukin-6 (IL-6), Myeloperoxidase (MPO), Toll-like receptor- 4 (TLR-4), Tumor necrosis factor-alpha (TNF-α).
Abstract:
Background: Cinnamic acid, an active compound in cinnamon spp., has anti-inflammatory
and antioxidant characteristics and is favorable in managing inflammatory bowel diseases.
Objectives: Evaluate cinnamic acid's effects on colitis in rats.
Methods: To induce colitis in experimental rats, excluding the sham group, a 4% intrarectal solution
of acetic acid was administered. The rats were then given oral doses of cinnamic acid at 30,
45, and 90 mg/kg for two days. The animals were assessed for macroscopic and microscopic
changes, and the levels of inflammatory mediators such as tumor necrosis factor-alpha (TNF-α),
interleukin-6 (IL-6), and myeloperoxidase (MPO) were measured using Eliza kits. Additionally,
real-time PCR was performed to examine the gene level of toll-like receptor 4 (TLR-4) in the
colon.
Results: Effective reduction of inflammation in acetic acid-induced colitis was achieved through
Cinnamic acid administration at doses of 45 and 90 mg/kg. The decrease was achieved by inhibiting
the activities of TNF-α, IL-6, and MPO while downregulating the expression of TLR-4. It is
important to note that macroscopic and microscopic evaluations were significant in determining
the effectiveness of cinnamic acid in reducing inflammation.
Conclusion: Downregulation of inflammatory cytokines and TLR-4 expression may contribute to
cinnamic acid's anti-inflammatory effect.
