Title:N-Stearoylethanolamine Exerts Cardioprotective Effects in Old Rats
Volume: 17
Issue: 2
Author(s): Halyna Kosiakova*, Andrii Berdyshev, Tetyana Horid’ko, Olena Meged, Vitaliy Klimashevsky, Roza Matsokha, Oksana Tkachenko, Valentina Asmolkova, Tetyana Kvitnitskaya-Ryzhova, Serhii Luhovskyi, Pavlo Klymenko and Nadiya Hula
Affiliation:
- OV Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Leontovich Str, 9, Kyiv, 03160,
Ukraine
Keywords:
N-stearoylethanolamine, aging, antioxidant enzymes, lipid peroxidation, proinflammatory cytokines, nitric oxide, phospholipids, cholesterol, rats.
Abstract:
Background: Aging is associated with the slowing down of metabolic processes, diminished
physiological processes, changes in hormonal activity and increasing exposure to oxidative
stress factors and chronic inflammation. The endocannabinoid system (ECS) is a major
signaling network that plays a pro-homeostatic role in the central and peripheral organs of the
human body. A class of minor lipids, N-acylethanolamines (NAEs), which do not activate cannabinoid
receptors, except for anandamide, but can potentiate the action of endocannabinoids
and have a wide spectrum of biological activity and significant adaptogenic potential, belongs to
ECS. The results of different studies over the past decades have established the protective effect
of NAE on many pathological conditions.
Objective: This study aimed to investigate the cardioprotective effects of C18:0
NAE— N-stearoylethanolamine (NSE) in aged rats. In this study, we focused on investigating
the effects of C18:0 NAE— N-stearoylethanolamine (NSE) on the intensity of oxidative/
nitrosative stress, antioxidant potential, lipoprotein profile and inflammation markers of
blood plasma, phospholipid composition and age-related morphological changes of old rat heart
tissues.
Methods: The study was conducted on Sprague Dawley male laboratory rats. The three groups of
rats were involved in the study design. The first group consisted of young rats aged 4 months
(n=10). The second (n=10) and third (n=10) groups included old rats aged of 18 months. Rats from
the third group were administered a per os aqueous suspension of NSE at a dose of 50 mg/kg of
body weight daily for 10 days. All groups of rats were kept on a standard vivarium diet. The blood
plasma, serum, and heart of rats were used for biochemical and histological analysis.
Results: The cardioprotective effect of N-stearoylethanolamine in old rats was established,
which was expressed in the normalization of the antioxidant system condition and the level of
proinflammatory cytokines, positive modulation of blood plasma and lipoprotein profile, normalization
of heart tissue lipid composition, and significant reduction in age-related myocardium
morphological changes.
Conclusion: The revealed effects of N-stearoylethanolamine can become the basis for developing
a new drug for use in complex therapy to improve the quality of life of older people.
