Title:Jeduxiaoliu Formula can Induce Apoptosis of Lymphoma Cells In Vitro and In Vivo
Volume: 28
Issue: 2
Author(s): XiaoWei Feng, Ming Hu, Cong Ming Wei, Hong Yu Zhang, Wen Wen Jiang and Qi Hu*
Affiliation:
- Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine,
Shanghai, 200071, China
Keywords:
Jeduxiaoliu formula, RNA-Seq, apoptosis, NF-κB signalling pathway, TNF signalling pathway, lymphoma.
Abstract:
Objective: Lymphoma is the most common malignancy of the haematological system.
Jeduxiaoliu formula (JDXLF) exerts good therapeutic effects against lymphoma, however, the
mechanisms underlying these effects remain unclear. Therefore, this study aimed to investigate
the mechanism of action of JDXLF.
Method: RNA-Seq was performed to examine the molecular mechanisms underlying the therapeutic
effects of JDXLF against lymphoma. CCK-8 assay was performed to examine the effects
of JDXLF on the proliferation of lymphoma cells. Electron microscopy was performed to examine
the morphology of lymphoma cells. Flow cytometry was performed to examine the apoptosis and
cell cycle of lymphoma cells. qPCR and Western blotting were performed to detect the expression
of apoptotic genes and proteins. In vivo, the tumour-suppressive effect of JDXLF on lymphoma
transplanted tumours was examined by establishing a subcutaneous transplantation tumour model
in nude mice, and the expression of apoptotic proteins in tumour tissues was analysed via immunohistochemical
staining.
Results: RNA-Seq revealed 71, 350 and 620 differentially expressed genes (DEGs) in the 1
mg/mL, 4 mg/mL and 8 mg/mL JDXLF treatment groups, respectively. KEGG pathway analysis
showed that the DEGs were significantly associated with apoptosis, TNF signalling and NF-κB
signalling. In vitro experiments revealed that JDXLF inhibited the proliferation of lymphoma (Raji
and Jeko-1) cells in a dose-dependent manner, induced apoptosis and upregulated the expression
of Bax/Bcl2 and caspase3.In vivo experiments revealed that JDXLF had a significant tumourshrinking
effect on mice and increased the expression of the apoptosis-related proteins caspase3
and Bax/Bcl2.
Conclusions: This study indicates that JDXLF can induce apoptosis in lymphoma cells In vitro
and . We In vivo suggest this may provide a direction for further research into lymphoma therapy.
