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摘要
背景:自噬在肺鳞状细胞癌(LUSC)的发展中起着至关重要的作用。泛素特异性肽酶31 (USP31)最近被发现参与多种癌症的发展。然而,USP31是否调节LUSC中的自噬尚不清楚。 方法:本研究通过基因表达谱交互分析(GEPIA)数据库、定量实时PCR (qRT-PCR)和Western blot分析,发现LUSC组织样本中USP31表达水平较高。通过细胞计数试剂盒8 (CCK-8)检测细胞增殖和集落形成,表明usp31稳定敲除降低了细胞活力。 结果:免疫荧光分析表明,USP31敲低抑制了LUSC自噬的发生。同时,USP31通过蛋白酶体途径稳定E2F转录因子1 (E2F1)的表达。此外,过表达E2F1可有效消除USP31敲低对LUSC细胞增殖和自噬的影响。 结论:综上所述,本研究证明USP31促进LUSC细胞生长和自噬,至少部分是通过稳定E2F1的表达,为LUSC的治疗提供了潜在的治疗基因。
关键词: 肺鳞癌,增殖,泛素特异性肽酶31,E2F转录因子1,肺癌,非小细胞肺癌。
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