Title:Human Adipose-derived Stem Cells Upregulate IGF-1 and Alleviate Osteoarthritis in a Two-stage Rabbit Osteoarthritis Model
Volume: 19
Issue: 11
Author(s): Juan Wang*, Shibo Su, Chuanming Dong, Qiang Fan, Jishu Sun, Siqiang Liang, Zuhuo Qin, Chuqing Ma, Jianfeng Jin, Hongwen Zhu*, Tongmeng Jiang*Jun Xu*
Affiliation:
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, 571199, China
- Engineering Research Center for Hainan Bio-Smart Materials and
Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan
Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University,
Haikou, 571199, China
- Stem Cell Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai,
200120, China
- Orthopedics Department, Tianjin Hospital, Tianjin, 300000, China
- Engineering Research Center for Hainan Bio-Smart Materials and
Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan
Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University,
Haikou, 571199, China
- Stem Cell Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai,
200120, China
Keywords:
Human adipose-derived stem cells, osteoarthritis, rabbit, IGF-1, ELISA, qRT-PCR techniques.
Abstract:
Objective: In recent times, it has been recognized that mesenchymal stem cells (MSCs)
possess the capability to address osteoarthritis (OA). The objective of this research was to examine
the impact of injecting human adipose-derived stem cells (hADSCs) into a novel rabbit osteoarthritis
model with dual damage.
Methods: The OA model was established surgically first by medial collateral ligament and anterior
cruciate ligament transection and medial meniscectomy, then by articular cartilage full-thickness
defect. Enhanced Green Fluorescence Protein expressing lentivirus FG12 was used to label
hADSCs, which were then injected into the knee joints. Every single rabbit was sacrificed after 4
and 8 weeks following the surgical procedure. Macroscopic examination, immunohistochemistry
staining, magnetic resonance imaging, qRT-PCR, and ELISA analysis were utilized for the assessments.
Results: After 4 and 8 weeks, the injection of hADSCs resulted in reduced cartilage loss, minimal
fissures and cracks, and a decrease in the volume of joint effusion and cartilage defect as measured
by MRI. Moreover, the application of ELISA and qRT-PCR techniques revealed that the administration
of hADSCs resulted in an elevation in the IGF-1 concentration.
Conclusions: Based on our findings, it can be inferred that the transplantation of hADSCs facilitates
the healing of articular cartilage in the osteoarthritis model of rabbits with double damage.
The upregulated IGF-1 may play a crucial part in the process of cartilage repair using hADSCs.
The use of hADSC transplantation could potentially be appropriate for clinical implementation in
managing osteoarthritis.
