Title:Protein Profiling of Hirudo orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy
Volume: 20
Issue: 3
Author(s): Leili Amani, Mehran Mirabzadeh Ardakani, Nasrin Motamed, Masuomeh Malek and Marzieh Dehghan Shasaltaneh*
Affiliation:
- Department of Biology, Faculty of Science, University of Zanjan, Zanjan, Iran
Keywords:
Disease, treatment, H. orientalis, medicinal leech, protein profiling, seasons.
Abstract: Objective: Leech therapy has been used for centuries as a recommended approach to
cure several diseases, such as; psoriasis, arthrosclerosis, urinary tract diseases, and wound healing.
The present study aimed to analyze the number, quantity, and distribution differences of medicinal
leech (Hirudo orientalis) proteins throughout various seasons and in laboratory conditions as well.
Methods: Protein profiling of salivary gland secretion from leech was studied by SDS-PAGE and
2D Electrophoresis on the proteins with the molecular weight range of 5 - 250 KDa in the lyophilized
salivary gland secretion (SGS) during the seasons of summer and winter, and also in the laboratory
conditions.
Results: Our results indicated differences in the number and quality of leech saliva proteins in different
seasons. We observed a higher number of proteins in summer than in winter. These results
demonstrated the presence of Calin and Manillase in summer and Hyaluronidase and Collagenase
in winter.
Conclusion: This study could help us in choosing the best and most favorable conditions for using
H. orientalis proteins for the treatment of different diseases.