Title:Bioinformatics and Experimental Study Revealed LINC00982/
miR-183-5p/ABCA8 Axis Suppresses LUAD Progression
Volume: 24
Issue: 6
Author(s): Defang Ding*, Jingyu Zhong, Yue Xing, Yangfan Hu, Xiang Ge and Weiwu Yao*
Affiliation:
- Department of Imaging, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336,
China
- Department of Imaging, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336,
China
Keywords:
Lung adenocarcinoma (LUAD), LINC00982, miR-183-5p, ATP binding cassette subfamily a member 8 (ABCA8), ceRNA, bioinformatics.
Abstract:
Background: Lung adenocarcinoma (LUAD) is a major health challenge worldwide
with an undesirable prognosis. LINC00982 has been implicated as a tumor suppressor in diverse
human cancers; however, its role in LUAD has not been fully characterized.
Methods: Expression level and prognostic value of LINC00982 were investigated in pan-cancer
and lung cancer from The Cancer Genome Atlas (TCGA) project. Differential expression analysis
based on the LINC00982 expression level was performed in LUAD followed by gene set enrichment
analysis (GSEA) and functional enrichment analyses. The association between LINC00982
expression and tumor immune microenvironment characteristics was evaluated. A potential ceRNA
regulatory axis was identified and experimentally validated.
Results: We found that LINC00982 expression was downregulated and correlated with poor prognosis
in LUAD. Enrichment analyses revealed that LINC00982 could inhibit DNA damage repair
and cell proliferation, but enhance tumor metabolic reprogramming. We identified a competing endogenous
RNA network involving LINC00982, miR-183-5p, and ATP-binding cassette subfamily
A member 8 (ABCA8). Luciferase assays confirmed that miR-183-5p can interact with
LINC00982 and ABCA8. Forced miR-183-5p expression reduced LINC00982 transcript levels
and suppressed ABCA8 expression.
Conclusions: Our findings revealed the LINC00982/miR-183-5p/ABCA8 axis as a potential therapeutic
target in LUAD.