Title:Compression Promotes the Osteogenic Differentiation of Human
Periodontal Ligament Stem Cells by Regulating METTL14-mediated
IGF1
Volume: 19
Issue: 8
Author(s): Zengbo Wu*
Affiliation:
- North Sichuan Medical College, Xinglin Community, Sihai Street, Shunqing District, Nanchong, Sichuan, 637000,
China
Keywords:
Orthodontic treatment, METTL14, IGF1, osteogenic differentiation, hPDLSCs, periodontal ligament, alkaline phosphatase.
Abstract:
Background and Objectives: Orthodontic treatment involves the application of mechanical
force to induce periodontal tissue remodeling and ultimately promote tooth movement. It is essential
to study the response mechanisms of human periodontal ligament stem cells (hPDLSCs) to
improve orthodontic treatment.
Methods: In this study, hPDLSCs treated with compressive force were used to simulate orthodontic
treatment. Cell viability and cell death were assessed using the CCK-8 assay and TUNEL staining.
Alkaline phosphatase (ALP) and alizarin red staining were performed to evaluate osteogenic
differentiation. The binding relationship between IGF1 and METTL14 was assessed using RIP
and dual-luciferase reporter assays.
Results: The compressive force treatment promoted the viability and osteogenic differentiation of
hPDLSCs. Additionally, m6A and METTL14 levels in hPDLSCs increased after compressive
force treatment, whereas METTL14 knockdown decreased cell viability and inhibited the osteogenic
differentiation of hPDLSCs treated with compressive force. Furthermore, the upregulation
of METTL14 increased m6A levels, mRNA stability, and IGF1 expression. RIP and dual-luciferase
reporter assays confirmed the interaction between METTL14 and IGF1. Furthermore, rescue
experiments demonstrated that IGF1 overexpression reversed the effects of METTL14 knockdown
in hPDLSCs treated with compressive force.
Conclusions: In conclusion, this study demonstrated that compressive force promotes cell viability
and osteogenic differentiation of hPDLSCs by regulating IGF1 levels mediated by METTL14.
