Title:Mesenchymal Stromal Cells Nanovesicles Carry microRNA with Nephroprotective Proprieties Regardless of Aging
Volume: 17
Issue: 2
Author(s): Marcia Bastos Convento*, Andréia Silva de Oliveira, Mirian Aparecida Boim and Fernanda Teixeira Borges
Affiliation:
- Nephrology Division, Department of Medicine, Federal University of Sao Paulo, Street Borges Lagoa, 783, 6º Floor,
Doctors' Building, Sao Paulo - SP - CEP 04038-901, Brazil
Keywords:
Aging, extracellular vesicles, mesenchymal stromal cells, nephrotoxicity injury, paracrine mechanism, umbilical cord.
Abstract:
Containing information molecules from their parent cells and inclining to fuse with
targeted cells, bone marrow mesenchymal stromal cells-derived extracellular vesicles (MSCs-
EV) are valuable in nanomedicine.
Background: The effects of aging on the paracrine mechanism and in the production and action
of MSCs-EV and their cargos of miR-26a and siRNA-26a for the treatment of tubular renal cells
under nephrotoxicity injury remain unelucidated.
Objective: The purpose of this study was to evaluate MSCs-EV of different ages and their ability
to deliver the cargos of miR-26a and siRNA-26a to target renal tubular cells affected by nephrotoxicity
injury.
Methods: In a model of gentamicin-induced nephrotoxicity, renal tubular cells treated with
MSCs-EV expressing or not expressing microRNA-26a were analyzed. Western blotting was utilized
to evaluate cell cycle markers, and MTT assay was utilized to evaluate auto-renovation
capacity.
Results: Tubular cells under nephrotoxicity injury showed decreased proliferative capacity, but
the treatment in the tubular renal cells under nephrotoxicity injury with MSCs-EV expressing
microRNA-26a showed nephroprotective effects, regardless of EV age. While the treatment with
EV-mediated siRNA-26a failed to preserve the nephroprotective effects equally, regardless of
age.
Conclusion: Mesenchymal stromal cell nanovesicles carry microRNA with nephroprotective proprieties
regardless of aging.
