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Research Article

LIMD2是急性心肌梗死中细胞衰老-免疫/炎症的标志

卷 31, 期 17, 2024

发表于: 02 November, 2023

页: [2400 - 2413] 页: 14

弟呕挨: 10.2174/0109298673274563231031044134

价格: $65

摘要

背景:急性心肌梗死(AMI)是一种年龄依赖性心血管疾病，其中细胞老化、免疫和炎症因素改变病程;然而，AMI的细胞老化-免疫/炎症特征尚未研究。 方法:基于GEO数据库获取microRNA (miRNA)测序、mRNA测序和单细胞测序数据，利用Seurat软件包对ami相关细胞亚群进行鉴定。随后，筛选差异表达的mirna和mrna，建立竞争内源性rna (ceRNAs)网络。通过单样本基因集富集分析(ssGSEA)、ESTIMATE和CIBERSORT算法计算衰老和免疫评分，并使用Hmisc包筛选与衰老和免疫评分相关性最高的基因。最后，通过蛋白-蛋白相互作用(PPI)和分子对接分析预测AMI治疗的潜在药物。 结果:AMI中有4种细胞类型(巨噬细胞、成纤维细胞、内皮细胞、CD8 T细胞)，其中CD8 T细胞衰老活性最低。基于差异表达miRNAs (DEmiRNAs)靶基因和差异表达mrna (demmrnas)中的重叠基因，建立了miRNAs- mNRA相互作用的ceRNA网络。观察CD8 T细胞的24个标记基因。LIMD2被认为是AMI中细胞衰老免疫/炎症相关的中枢基因。本研究还发现了DB03276-LIMD2- ami的潜在治疗网络，该网络在DB03276-LIMD2之间表现出优异且稳定的结合状态。 结论:本研究确定LIMD2是细胞衰老免疫/炎症相关的中枢基因。ceRNA网络和DB03276-LIMD2-LAMI治疗网络丰富了对AMI发病机制和治疗机制的认识。

关键词: 急性心肌梗死，细胞老化，免疫，炎症，cerna, LIMD2。

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