Title:Design and Simulation of the Microcantilever Biosensor for MITF Antigen
and D5 Monoclonal Antibody Interaction Finite Element Analysis, and
Experimental
Volume: 25
Issue: 3
Author(s): Pelin Akcali, Kübra Kelleci*Sevil Ozer
Affiliation:
- Department of Medical
Services and Techniques, Beykoz Vocational School of Logistics, Istanbul, Turkey
Keywords:
Resonance frequency, mode shapes, finite element analysis, antibody-antigen interaction, AFM cantilever, anti- MITF antibody.
Abstract:
Background: Biosensors and MEMS have witnessed rapid development and enormous
interest over the past decades. Constant advancement in diagnostic, medical, and chemical applications
has been demonstrated in several platforms and tools. In this study, the analytical and FEA
of the microcantilever used in biomolecular analyses were compared with the experimental analysis
results.
Methods: In this study, MITF antigen, which is a melanoma biomarker, and anti-MITF antibody
(D5) were selected as biomolecules. A MEMS-type microcantilever biosensor was designed by
functionalizing the AFM cantilever by utilizing the specific interaction dynamics and intermolecular
binding ability between both molecules. Surface functionalization of cantilever micro biosensors
was performed by using FEA. The stress that will occur as a result of the interactions between
the MITF-D5 has been determined from the deviation in the resonant frequency of the cantilever.
Results: It has been found that the simulation results are supported by analytical calculations and
experimental results.
Conclusion: The fact that the results of the simulation study overlap with the experimental and
mathematical results allows us to get much cheaper and faster answers compared to expensive and
time-consuming experimental approaches.