Title:Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols:
A Guideline for Regenerative Medicine
Volume: 19
Issue: 7
Author(s): Priscila Elias Ferreira Stricker, Nathalia Barth de Oliveira, Bassam Felipe Mogharbel, Larissa Lührs, Ana Carolina Irioda, Eltyeb Abdelwahid, Luciane Regina Cavalli, Idiberto José Zotarelli-Filho*Katherine Athayde Teixeira de Carvalho*
Affiliation:
- Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties, Curitiba, Brazil
- ABRAN - Associação
Brasileira de Nutrologia/Brazilian Association of Nutrology, Catanduva, Sao Paulo, Brazil
- College of Palliative
Medicine of Sri Lanka, Colombo, Sri Lanka
- Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties, Curitiba, Brazil
Keywords:
Mesenchymal stem cells, immortalization, protocols, tumorigenicity test, systematic review, meta-analysis.
Abstract:
Background: This systematic review describes the most common methodologies for immortalizing
human and animal mesenchymal stem cells (MSCs). This study follows the rules of
PRISMA and is registered in the Institutional Review Board of PROSPERO International of systematic
reviews, numbered protocol code: CRD42020202465.
Method: The data search systematization was based on the words “mesenchymal stem cell” AND
“immortalization.” The search period for publications was between 2000 and 2022, and the databases
used were SCOPUS, PUBMED, and SCIENCE DIRECT. The search strategies identified 384 articles:
229 in the SCOPUS database, 84 in PUBMED, and 71 in SCIENCE DIRECT. After screening
by titles and abstracts, 285 articles remained. This review included thirty-nine articles according to
the inclusion and exclusion criteria.
Result: In 28 articles, MSCs were immortalized from humans and 11 animals. The most used immortalization
methodology was viral transfection. The most common immortalized cell type was the
MSC from bone marrow, and the most used gene for immortalizing human and animal MSCs was
hTERT (39.3%) and SV40T (54.5%), respectively.
Conclusion: Also, it was observed that although less than half of the studies performed tumorigenicity
assays to validate the immortalized MSCs, other assays, such as qRT-PCR, colony formation in
soft agar, karyotype, FISH, and cell proliferation, were performed in most studies on distinct MSC
cell passages.
