Title:Effect of Surfactant Concentration on Physicochemical and Antibacterial
Properties of Eugenol Nanoemulsions
Volume: 2
Author(s): Indra Bhusan Basumatary and Santosh Kumar*
Affiliation:
- Department of Food Engineering and Technology, Central Institute of Technology Kokrajhar, Kokrajhar 783370, Assam, India
Keywords:
Nanoencapsulation, Tween 80, Antimicrobial activity, Antioxidant activity, Stability of nanoemulsion, EuNEs.
Abstract:
Introduction:
Nanoencapsulation has gained popularity in recent decades due to its numerous benefits, such as improved stability, oxidation resistance, retention
of volatile ingredients, controlled release, delivery of multiple active ingredients in a row, improvement in organoleptic properties, reduction of
toxic side effects, and water solubility.
Methods:
The present study was aimed to prepare eugenol nanoemulsions (EuNEs) using non-ionic surfactant (Tween 80) by ultrasound-assisted techniques
and to evaluate the effects of surfactant concentration on their droplets size, polydispersity index (PDI), zeta potential, storage stability,
antioxidant, and antibacterial activities. Antimicrobial activity of the prepared nanoemulsions was tested against Gram-positive; Staphylococcus
aureus, Bacillus subtilis and Gram-negative; Escherichia coli, and Alcaligenes faecalis bacteria using well diffusion method.
Results:
The results showed that the droplet size decreased after a threshold Tween 80 concentration (10%), while PDI value increased with the increase in
surfactant concentration (Tween 80).
Conclusion:
The prepared EuNEs exhibited good antibacterial activity against all the four bacterial strains: E. coli, A. faecalis, S. aureus, and B. subtilis.