Title:Stem Cell-Based or Cell-Free Gene Therapy in Chondrocyte
Regeneration: Synovial Fluid-Derived Mesenchymal Stem Cell
Exosomes
Volume: 24
Issue: 7
关键词:
软骨缺损，滑膜液间充质干细胞，外泌体，软骨形成，hsa-miR-155-5p，胎儿人成软骨细胞系。
摘要:
Background: Cartilage injuries are currently the most prevalent joint
disease. Previous studies have emphasized the use of stem cells as the effective
treatment for regenerating cartilage damage.
Objective: In this study, considering the difficulties of the cellular therapy method, it
was hypothesized that human synovial fluid-derived mesenchymal stem cell (hSFMSC)
exosomes as a SC source could be used to treat these injuries as a safer and
cell-free therapeutic alternative procedure due to its direct relevance to cartilage
regeneration. Moreover, this study aimed to determine the miRNA and target genes
required for the formation of SC treatment combined with gene therapy in order to
reveal the mechanism of cartilage regeneration and increase its effectiveness.
Methods: MSCs were characterized by flow cytometry, and immunocytochemical and
differentiation analyses were done. To characterize functionally isolated exosomes, in
vitro uptake analysis was performed. RT-qPCR was used to examine in terms of the
advantages of cellular and cell-free therapy, mature human chondroblasts derived by
differentiation from hSF-MSCs and human chondrocyte profiles were compared in
order to demonstrate the above profile of hSF-MSCs and exosomes isolated from
them, and the effectiveness of SC therapy in repairing cartilage damage.
Results: According to our findings, the expression level of hsa-miR-155-5p was found
to be considerably higher in chondrocytes differentiated from human synovial fluid
MSCs than in mature human chondrocytes. These findings were also supported by the
TGF-signalling pathway and chondrogenesis marker genes.
Conclusion: It was concluded that hSF-MSCs and exosomes can be used in the
treatment of cartilage damage, and hsa-miR-155-5p can be used as a target miRNA in
a new gene therapy approach because it increases the therapeutic effect on cartilage
damage.
