Title:Untargeted Metabolomic Analyses of Body Fluids to Differentiate
TBI DOC and NTBI DOC
Volume: 24
Issue: 9
关键词:
DOC, TBI, NTBI，代谢组学，生物标志物，坏死。
摘要:
Objective: To investigate the metabolomic differences between Traumatic
brain injury (TBI) disorder of consciousness (DOC) patients and non-traumatic brain
injury (NTBI) DOC patients by using cerebrospinal fluid (CSF), serum and urine
samples beneficial to understand the pathological mechanism differences between the
two etiologies, provide potential clues for the subsequent treatment and prognosis, and
investigate the metabolome differences and similarities between TBI and NTBI among
three different body fluids.
Methods: In total, 24 TBI DOC subjects and 29 NTBI DOC subjects were enrolled.
CSF, serum and urine samples from TBI DOC and NTBI DOC patients were collected
and analyzed by performing UPLC-MS. The statistical methods and pathway analyses
were applied to discover potential biomarkers and altered metabolic functions.
Results: When comparing TBI DOC and NTBI DOC, 36, 31 and 52 differential
metabolites were obtained in CSF, serum and urine, respectively. The functional
analysis of differential metabolites obtained in CSF, serum and urine were all related to
amino acid metabolism. Except for amino acid metabolism, metabolic biomarkers in
CSF, serum and urine mainly focus on central function, cognitive function, necrosis
and apoptosis and neurological function, respectively. In CSF, the highest AUC was
0.864 (Isoproturon) and 0.816 (Proline betaine). Then, the AUC of NFurfurylformamide
in serum was 0.941, while the AUC of Dihydronepetalactone and
Doxepin N-oxide glucuronide were 1.0 in urine.
Conclusion: CSF, serum and urine metabolomic analyses could differentiate TBI
DOC from NTBI DOC and functional analyses showed a metabolic change difference
between TBI DOC and NTBI DOC.
