Title:Efficient Hydrolysis of Cottonseed Oil with Amano Lipase Entrapped on Hydrolysed
Polyvinyl Alcohol Gel Pellets Optimized via Factorial Design
Volume: 27
Issue: 18
Author(s): Farah Naz Talpur*, Sidra Khan, Ahsanullah Unar, Hassan Imran Afridi, Adnan Ibrahim and Muhammad Sohail Bashir*
Affiliation:
- National Center of Excellence in Analytical Chemistry, University of Sindh, 76080, Jamshoro, Pakistan
- Hefei National Laboratory for Physical Sciences at the Microscale, CAS
Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of
China, Hefei, Anhui 230026, China
- Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education,
Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China
Keywords:
Cottonseed oil, amano lipase, polyvinyl alcohol gel/pellets, factorial design, optimization, immobilization.
Abstract:
The aim of this study was to obtain the optimum hydrolysis of cottonseed oil via immobilized lipases
(Lipases Rhizopus oryzae, SL from Pseudomonas (Burkholderia) cepacia, Amano lipase from Mucor javanica,
ANL from Aspergillus niger lipase, TL from Pseudomonas stutzeri, QLM from Alcaligenes sp., Triacylglycerol
lipase (EC 3.1.1.3), PL from Alcaligenes sp., AL from lysosomal acid lipase, Candida antarctica (CA), and Candida
cylindrea (CC)) on hydrolysed polyvinyl alcohol (PVA) gel pellets.
The production of free fatty acids by the hydrolysis of triglycerides from several sources is an important component
in the economic exploitation of naturally produced renewable raw materials.
Herein, various lipases, including Rhizopus oryzea, SL, Amano lipase from Mucor javanica, ANL, TL, QLM, EC,
PL, AL, CA, and CC were screened for optimum hydrolysis of cottonseed oil. Following the selection of lipase for
hydrolysis of cottonseed oil, transparent PVA gel was synthesized by physical gelation method employing Dimethyl
sulfoxide and a water system. In the last step, immobilization of selected lipase i.e. Amano lipase for cotton
seed oil was carried out at 4oC for one hour. After successful synthesis and immobilization, the PVA gel was characterized
by FTIR and SEM analysis. Finally, factorial design was employed for optimization of experimental variables
such as pH, time and amount of cotton seed oil hydrolysis by lipase immobilized PVA.
The obtained results indicated that lipase Amano was found better with higher free fatty acids (FFA) yields (with
98.38%) in 5 hours using 3 g of cottonseed oil, suggesting that PVA immobilized Amano lipase is an efficient catalyst
to hydrolyze the cottonseed oil.
The overall study indicated that hydrolysed PVA gel is an effective biocompatible solid support for immobilization
of lipases to obtain higher FFA from cottonseed oil following hydrolysis reaction.
