Title:Overexpression of SOCS2 Inhibits EMT and M2 Macrophage Polarization
in Cervical Cancer via IL-6/JAK2/STAT3 Pathway
Volume: 27
Issue: 7
Author(s): Dan Li, Yandan Huang, Min Wei, Bin Chen and Yan Lu*
Affiliation:
- Department of Gynecologic Oncology, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, 530021, People’s
Republic of China
Keywords:
Cervical cancer, SOCS2, IL-6, JAK2/STAT3, EMT, M2 macrophage.
Abstract:
Objective: SOCS2 is a member of the suppressor of cytokine signaling (SOCS) protein
family associated with the occurrence and development of multiple cancers. This study revealed
the expression and molecular mechanisms of SOCS2 in cervical cancer.
Methods: In this study, RT-qPCR, Western Blot, and immunohistochemistry were used to detect
the expression level of SOCS2 in cervical cancer tissues and tumor cells. We overexpressed
SOCS2 in SiHa cells via lentivirus. In-vitro experiments were used to investigate the changes in
cervical cancer cell proliferation, migration, and invasion ability before and after SOCS2 overexpression.
Western Blot was used to detect the expression of IL-6/JAK2/STAT3 pathway and EMTrelated
proteins. M0 macrophages were co-cultured with the tumor-conditioned medium. The
effect of SOCS2 on macrophage polarization was examined by RT-qPCR.
Results: SOCS2 expression level was significantly downregulated in cervical cancer tissues.
SOCS2 was negatively correlated with CD163+M2 macrophages. Overexpression of SOCS2 inhibited
the proliferation, migration, and invasion of cervical cancer cells. The expressions of Twist-
2, N-cadherin, and Vimentin were decreased, while the expression of E-cadherin was increased.
Moreover, the expression of IL-6, p-JAK2, and p-STAT3 were decreased. After the addition of
RhIL-6, the expression of E-cadherin protein in the LV-SOCS2 group was reversed. CM in the
LV-SOCS2 group inhibited the polarization of M2 macrophages.
Conclusion: SOCS2 acts as a novel biological target and suppressor of cervical cancer through IL-
6/JAK2/STAT3 pathway.