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Current Chinese Science


ISSN (Print): 2210-2981
ISSN (Online): 2210-2914

Research Article Section: Pharmacology

Identification of Active Compounds of Pueraria Lobata and their Mechanisms of Action by LC-MS/MS and Network Pharmacology

Author(s): Dong-min Cao* and Tao Liu

Volume 3, Issue 5, 2023

Published on: 19 September, 2023

Page: [398 - 406] Pages: 9

DOI: 10.2174/2210298103666230816090702


Background: Osteoporosis has become a global public health problem, and Pueraria Lobata (PL) is a potential drug for treating osteoporosis.

Methods: Firstly, the components of PL were detected and identified based on UHPLC-Q-Exactive Orbitrap MS. In addition, we used network pharmacology to study the potential mechanism of PL in treating osteoporosis.

Result: A total of 48 compounds, including 38 isoflavones, 6 puerosides, and 4 others, were identified by UHPLC-Q Exactive-Orbitrap MS. The network of the pharmacological analysis revealed that 28 compounds of PL regulated 19 pathways through 27 targets, including estrogen signaling pathway and NF-kappa B signaling pathway, etc.

Conclusion: This study used LC-MS combined with network pharmacology to identify the compounds of PL and their mechanism of intervention in osteoporosis, which provides a scientific basis for PL to become a functional food for preventing osteoporosis.

Keywords: Pueraria lobata, osteoporosis, UHPLC-Q exactive-orbitrap MS, network pharmacology, compounds, NF-kappa B signaling pathway.

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