Title:Identification of Active Compounds of Pueraria Lobata and their Mechanisms
of Action by LC-MS/MS and Network Pharmacology
Volume: 3
Issue: 5
Author(s): Dong-min Cao*Tao Liu
Affiliation:
- Translational Medicine Research Institute, First People’s Hospital of Foshan, Guangdong 528000, China
Keywords:
Pueraria lobata, osteoporosis, UHPLC-Q exactive-orbitrap MS, network pharmacology, compounds, NF-kappa B signaling pathway.
Abstract:
Background: Osteoporosis has become a global public health problem, and Pueraria Lobata
(PL) is a potential drug for treating osteoporosis.
Methods: Firstly, the components of PL were detected and identified based on UHPLC-Q-Exactive
Orbitrap MS. In addition, we used network pharmacology to study the potential mechanism of PL in
treating osteoporosis.
Result: A total of 48 compounds, including 38 isoflavones, 6 puerosides, and 4 others, were identified
by UHPLC-Q Exactive-Orbitrap MS. The network of the pharmacological analysis revealed
that 28 compounds of PL regulated 19 pathways through 27 targets, including estrogen signaling
pathway and NF-kappa B signaling pathway, etc.
Conclusion: This study used LC-MS combined with network pharmacology to identify the compounds
of PL and their mechanism of intervention in osteoporosis, which provides a scientific basis
for PL to become a functional food for preventing osteoporosis.
