Title:Optimization of Synthesis of Microbial Bio-surfactant Sophorolipids using Response Surface Methodology
Volume: 18
Issue: 2
Author(s): Priti Borde, Ninad Mhatre, Subhalaxmi Pradhan*Chandu S. Madankar*
Affiliation:
- Department of Basic Sciences, School of Basic and Applied Sciences, Galgotias University, UP, India
- Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Mumbai 400 019, India
Keywords:
Biosurfactants, sophorolipid, RSM, analysis, formulation, B glycosidic bond.
Abstract: Background: Synthetic surfactants, when released into the environment, do not degrade
completely and show harmful effects. To minimize the damage to the environment and to
introduce milder surfactants, it was necessary to introduce bio-surfactants.
Objective: Optimization of the yield is performed by using a design expert model. The
analysis of the product was carried out by using different techniques. The formulation of
the personal care product was prepared by using the sophorolipid produced.
Mode of Synthesis: Different compositions of raw materials are used, suggested by Design
expert software to optimize the yield of the sophorolipid. Fermentation was performed
by the shake flask method at specified conditions in the incubator shaker for the
synthesis. The extraction and separation of the sophorolipid were done by the solvent
extraction method.
Results: The predicted product yield value is close to the actual value of the product obtained,
which indicates the model is accurate to use. The effect of the raw materials on
the yield can be studied with the design expert model. The product is analyzed for its
composition and properties with different analysis methods.
Conclusion: Mathematical modelling is very helpful in predicting the optimum reaction
condition and improving the yield of a particular bio-process. The RSM model of design
expert software can be further utilized to carry out the in-detail study of the various factors
and their effect on optimizing the yield. The sophorolipid can be used in different
formulations as a greener and safe alternative to a chemical-based surfactant.
