Title:Regulatory Role, Mechanism, and Metabolic Profile of BIOTIN in
Gene Expression
Volume: 20
Issue: 2
Author(s): Ankita Wal, Abhijit Sasmal, Riya Singh, Princy Yadav, Yogesh Singh, Vipin Garg and Pranay Wal*
Affiliation:
- Department of Pharmacy, Pranveer Singh Institute of Technology, NH2 Kanpur Agra Highway, Bhauti, Kalyanpur, Kanpur Nagar, UP, India
Keywords:
Biotin, gene-expression, cell differentiation, spermatogenesis, neurotropic factor, glucose enzymes, glucokinase metabolism, cytokines.
Abstract: Biotin, a vitamin that is water-soluble, is part of the vitamin B complex and is required
by all living things, including humans. Biotin-dependent carboxylases are a prosthetic
group of enzymes, and biotin catalyzes essential processes in the production of fatty acids,
the breakdown of amino acids, and gluconeogenesis in eukaryotic cells. The role of biotin
as the prosthetic group of the four biotin-dependent carboxylases is well understood in
higher animals. Based on the roles of these carboxylases in metabolism, it was discovered
that biotin is required for cell survival, proliferation, and differentiation. Biotin appears to
play a role in cell function and has a spermatogenic impact. Biotin has been found to have a
direct impact on the transcription of important enzymes in glucose metabolism. Glucokinase
and phosphoenolpyruvate carboxykinase are glycolytic enzymes that biotin controls
(PEPCK). Biotin appears to be involved in gene control, which may explain some of its
functions regarding fetal development and cellular biology. According to investigations using
microarrays as well as other types of gene expression, biotin appears to affect the transcription
of genes encoding cytokines and their receptors, glucose metabolism genes, and
genes involved in cellular biotin homeostasis. A biotin shortage has a considerable effect on
gene expression in numerous tissues and cells, according to a microarray study. Biotin supplementation
affects the expression of several genes depending on the tissue, demonstrating
that gene expression differences reflect tissue function. Biotin affects energy, lipid, and glucose
metabolism, according to metabolite research, which has improved our understanding
of the biotin metabolic pathway. Using microarray and transcriptome analysis, this research
investigates the effect of biotin on gene expression.
