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Current Analytical Chemistry


ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Research Article

Use of Fe3O4@MPTMS-Dithizone Magnetic Nanoparticles as Solid Phase Sorbent for Sensitive Analysis of Sibutramine Molecules in Herbal Slimming Products

Author(s): Özge Demir, Halil İbrahim Ulusoy*, Ümmügülsüm Polat and Songül Ulusoy

Volume 19, Issue 6, 2023

Published on: 19 July, 2023

Page: [472 - 481] Pages: 10

DOI: 10.2174/1573411019666230703141135

Price: $65


Background: A new enrichment and sensitive determination method, which includes HPLCDAD analysis after Magnetic Solid Phase Extraction (MSPE), has been developed for trace analysis of Sibutramine molecules in herbal slimming products. Sibutramine is one of the most adulterated drug molecules in herbal products.

Method: In the proposed method, Sibutramine molecules were pre-concentrated by using Fe3O4@MPTMS-Dithizone magnetic sorbent synthesized in our laboratory. Desorption of Sibutramine molecules from the sorbent phase was carried out by using acetonitrile: methanol (1:1) solvent in the presence of pH 8.0 buffer before chromatographic determinations.

Results: Analytical parameters of the method, such as linear range, enrichment factor, and determination limit, were determined after optimizing experimental variables such as interaction time, desorption solvent, pH, etc. The sibutramine molecule was analyzed by isocratic elution of acetonitrile and KH2PO4 (pH 3.0, 0.05 M) (40:60) with a DAD detector at 223 nm wavelength. Limit of detection (LOD) value was calculated as 1.43 ng mL-1.

Conclusion: Relative standard deviations (RSD) were below 3.20% for determinations of model solutions, including 100 ng mL-1 of Sibutramine. Finally, the developed method has been applied to herbal slimming tea samples with quantitative recovery experiments.

Keywords: Sibutramine, magnetic solid phase extraction, HPLC, herbal slimming products, drug analysis, sibutramine molecule.

Graphical Abstract
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