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General Research Article

TRPM8作为通过文本挖掘和RNA测序相结合鉴定的胃癌的潜在生物标志物和治疗靶点

卷 23, 期 5, 2023

发表于: 13 June, 2023

页: [391 - 399] 页: 9

弟呕挨: 10.2174/1566523223666230529142423

价格: $65

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摘要

简介：胃癌是一种众所周知的恶性肿瘤，每年导致全球数百万人死亡。由于缺乏针对胃癌的特异性生物标志物，大多数患者在诊断时已处于疾病晚期，导致预后不良和死亡率较高。因此，迫切需要新的生物标志物来进行早期诊断并提高生存率。 方法：在本研究中，我们对 21 名胃癌患者的肿瘤样本进行了 RNA 测序。共鉴定出3192个差异表达基因（1589个上调基因和1603个下调基因）。随后，我们应用文本挖掘算法进一步分析这些数据，并选择 30 个代表性基因作为胃癌新型生物标志物的候选基因进行研究。 结果：在这些基因中，基于对 134 个样本进行的蛋白质印迹和免疫化学验证，我们确认瞬时受体电位褪黑素 8 信道 (TRPM8) 是一种新型生物标志物。与正常胃组织相比，肿瘤组织中TRPM8的表达水平显着升高。 结论：本研究深入了解 TRPM8 在细胞增殖中的潜在作用。此外，TRPM8可能作为胃癌患者的潜在治疗靶点。

关键词: TRPM8，胃癌，增殖，文本挖掘，生物标志物，癌基因。

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