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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Review Article

Protein Engineering, a Robust Tool to Engineer Novel Functions in Protein

Author(s): Nancy, Sudarshan Singh Lakhawat, Sanjeev Chandel, Sunil Kumar Jaswal and Pushpender Kumar Sharma*

Volume 30, Issue 7, 2023

Published on: 14 June, 2023

Page: [541 - 551] Pages: 11

DOI: 10.2174/0929866530666230519122612

Price: $65

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Abstract

Designing effective diagnostics, biotherapeutics, and biocatalysts are a few interesting potential outcomes of protein engineering. Despite being just a few decades old, the discipline of de novo protein designing has provided a foundation for remarkable outcomes in the pharmaceuticals and enzyme industries. The technologies that will have the biggest impact on current protein therapeutics include engineered natural protein variants, Fc fusion protein, and antibody engineering. Furthermore, designing protein scaffolds can be used in developing next-generation antibodies and in transplanting active sites in the enzyme. The article highlights the important tools and techniques used in protein engineering and their application in the engineering of enzymes and therapeutic proteins. This review further sheds light on the engineering of superoxide dismutase, an enzyme responsible for catalyzing the conversion of superoxide radicals to oxygen and hydrogen peroxide by catalyzing a redox reaction at the metal center while concurrently oxidizing and reducing superoxide free radicals.

Keywords: Protein engineering, superoxide dismutase, site-directed mutagenesis, therapeutics, catalytic, chemical.

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