Title:Early Markers of Sepsis Cardiomyopathy in Murine Models by
Echocardiography
Volume: 20
Author(s): Sanjeev Nirala*Xue Rui Tan
Affiliation:
- Department of Cardiovascular Medicine, First Affiliated Hospital of Shantou, University Medical College, Shantou, P.R. China
Keywords:
Sepsis cardiomyopathy, Echocardiography, Blood flow, CLP, Strain depression, Heart. Article
Abstract:
Background:
Strain echocardiography (SE) is a procedure for analyzing myocardial dysfunction that is known to be less dependent on preload and afterload of
heart function. Unlike dimension-based parameters, like ejection fraction (EF) and fractional shortening (FS), SE measures cardiac function by
tracking cardiac tissue deformation and anomalies throughout the cardiac cycle. Although SE is proven to locate myocardial ailments in various
heart diseases, few studies exist regarding using SE relevant to sepsis pathophysiology.
Objective:
The study aimed to calculate the myocardial strain and strain rates, like longitudianl strain (LS), global radial strain (GRS), and global longitudinal
strain (GLS), and to show these to be reduced earlier in cecal ligation and puncture (CLP) and lipopolysaccharide (LPS)-induced sepsis in
coordination with an elevation of pro-inflammatory cytokines.
Methodology:
Wild-type mice C57BL/6J (WT) were taken in this study and classified as CLP, LPS, and control groups. CLP surgery and LPS injection were
given to induce sepsis. Endotoxemic septic shock was induced by intraperitoneal (IP) injection of LPS Escherichia coli.
Echocardiography short axis views (SAX), longitudinal strain (LS), global circumferential strain (GCS), and global radial strain (GRS) were
measured from the anterior and posterior positions of the septal and lateral walls of the heart. Real-time polymerase chain reaction (RT-PCR) was
performed to evaluate post-CLP and LPS to analyze cardiac pro-inflammatory cytokines expressions. Inter- and intra-observer variables were
tested by Bland-Altman analyses (BA). All data analysis was performed by GraphPad Prism 6 software. p<0.05 was taken as statistically
significant.
Results:
After 48 hours following CLP and LPS-induced sepsis, a significant declination in both longitudinal strain and strain rate (LS and LSR) was
identified in the CLP and LPS groups compared to the control group. Strain depression in sepsis was linked with the up-regulation of proinflammatory
cytokines in RT-PCR analysis.
Conclusion:
In the present study, we found myocardial strain and strain rate parameters, like LS, GRS, and GLS, to be reduced after CLP and LPS-induced
sepsis in coordination with the elevation of pro-inflammatory cytokines.
