Title:Key Mutant Genes and Biological Pathways Involved in Aspirin Resistance
in the Residents of the Chinese Plateau Area
Volume: 27
Issue: 4
Author(s): Jinchun Wu*, Rong Chang*Yanmin Liu
Affiliation:
- Department of Cardiology, Qinghai Provincial People's Hospital, Xining, Qinghai, 810007, China
- Department of
Cardiology, Shenzhen Longhua District Central Hospital, The Affiliated Central Hospital of Shenzhen Longhua District,
Guangdong Medical University, Shenzhen, Guangdong, 518109, China
Keywords:
Aspirin resistance, plateau area, SNP, Arachidonic acid, pathway analysis, genotyping.
Abstract:
Introduction: Aspirin is used to prevent and treat cardiovascular diseases; however,
some patients develop aspirin resistance.
Aim: We aimed to explore the potential molecular mechanisms underlying aspirin resistance in
people living in the Chinese plateau area.
Methods: In total, 91 participants receiving aspirin treatment from the Qinghai plateau area were
divided into the aspirin resistance and aspirin sensitivity groups. Genotyping was performed using
the Sequence MASSarray. Differentially mutated genes between the two groups were analyzed using
MAfTools. The annotation of differentially mutated genes was conducted based on the
Metascape database.
Results and Discussion: In total, 48 differential SNP and 22 differential InDel mutant genes between
the aspirin resistance and aspirin sensitivity groups were screened using Fisher’s exact test
(P < 0.05). After the χ2 test, a total of 21 SNP mutant genes, including ZFPL1 and TLR3, and 19
InDel mutant genes were found to be differentially expressed between the two groups (P < 0.05).
Functional analysis revealed that these differential SNP mutations were mainly enriched in aspirin
resistance pathways, such as the Wnt signaling pathway. Furthermore, these genes were related to
many diseases, including various aspirin indications.
Conclusion: This study identified several genes and pathways that could be involved in arachidonic
acid metabolic processes and aspirin resistance progression, which will provide a theoretical
understanding of the molecular mechanism of aspirin resistance.