Title:Toxocara Canis Increases the Potential of Breast Cancer by
Reducing the Expression of the P53 Protein
Volume: 24
Issue: 3
关键词:
犬弓形虫，乳腺癌，细胞凋亡，血管生成，炎症，肿瘤大小。
摘要:
Introduction: Breast cancer is considered the most frequent type of cancer
in women with high mortality worldwide, and most importantly, it is the second most
common cancer. However, some breast cancer-related risk factors remain unknown.
So, the current study was designed to evaluate the effect of Toxocara canis on the
biomarkers correlated with proliferation, apoptosis, inflammation, and angiogenesis in
4T1 tumor-bearing mice infected with Toxocara canis for the first time.
Methods: Mice were categorized into four groups: A) control, B) treated with 4T1+
Toxocara canis, C) treated with Toxocara canis, and D) treated with 4T1. The expression
of Ki-67 and P53 was then evaluated by using the immunohistochemical technique.
In addition, the levels of transforming growth factor-β, Interferon gamma-γ, Interleukin
10, tumor necrosis factor-α and vascular endothelial growth factor as well as
anti- Toxocara canis IgG were determined using the enzyme-linked immunosorbent
assay method.
Results: The expression of Ki-67 was significantly increased in the 4T1+ Toxocara canis group than control and Toxocara canis groups (P < 0.001 and P < 0.001, respectively).
Moreover, a significant decrease in P53 was found in the 4T1+ Toxocara canis
group than in the control and Toxocara canis groups (P < 0.001 and P < 0.001, respectively).
Also, the 4T1+ Toxocara canis group significantly reduced the expression of
P53 more than 4T1 tumor-bearing mice (P = 0.005). In addition, the 4T1+ Toxocara
canis group had an increasing tumor necrosis factor-α and vascular endothelial growth
factor than controls (P = 0.004 and P = 0.002, respectively). Furthermore, a significant
reduction in Interleukin 10 was found in the 4T1+ Toxocara canis group than in the
control group (P = 0.004).
Conclusion: Our findings showed that Toxocara canis could probably increase the
potential of breast cancer by reducing P53 in 4T1 tumor-bearing mice infected with
Toxocara canis more than other groups.
