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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

The Metabolism of Portulacatone B from Portulaca oleracea L. in Rats by UHPLC-ESI-Q-TOF/MS

Author(s): Xinyu Cui, Xiujuan Lan, Aijing Leng* and Xixiang Ying*

Volume 19, Issue 4, 2023

Published on: 21 March, 2023

Page: [301 - 306] Pages: 6

DOI: 10.2174/1573412919666230306124751

Price: $65

Abstract

Objective: This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which is an alkaloid isolated from Portulaca oleracea L.

Methods: Portulacatone B was administered through the tail vein of the rat, and the orbital blood at 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathways in the rat were researched by ultra-high performance liquid chromatographyelectrospray coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF/ MS).

Results: The research results of the metabolites and metabolic pathways of Portulacatone B showed that after administration through the tail vein of rats, 3 metabolites were found in the plasma sample, 2 metabolites in the urine sample, and one metabolite in the feces sample. The main metabolic pathways were found to be oxidation, hydrolysis, methylation, glucuronidation, and sulfonation.

Conclusion: Six metabolites were found in the rat’s plasma, urine, and feces samples, and the metabolic pathways included oxidation, hydrolysis, methylation, glucuronidation, and sulfonation process.

Keywords: Portulaca oleracea L., Portulacatone B, metabolism, UHPLC-ESI-Q-TOF/MS, glucuronidation, flavonoids, plasma.

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