Title:Hydroalcoholic Extract of Achillea Wilhelmsii Decreases the Expressions
of Hippo Signaling Pathway-Associated Oncogenes in the A549 Lung
Cancer Cell Line
Volume: 17
Issue: 2
Author(s): Fariba Nabatchian, Maryam Davoudi, Mojtaba Ashtiani*, Negin Davoudi and Reza Afrisham
Affiliation:
- Department of Medical Laboratory Sciences, School of Allied Medicine, Tehran University of Medical Sciences, Tehran,
Iran
Keywords:
Lung cancer, Achillea wilhelmsii, hippo, pathway, A549, hydroalcoholic extract.
Abstract:
Background: Achillea wilhelmsii used in traditional Iranian medicine to treat a variety of
disorders, has been proven to contribute to some signaling pathways in cancers. Evidence suggests
that the Hippo pathway, which regulates organ size, is altered in a few conditions like lung cancer. In
this regard, this study aimed to evaluate the effect of the hydroalcoholic extract of this plant on the viability
and mRNA expression of some Hippo signaling pathway-associated oncogenes and suppressors
in A549 lung cancer cell lines.
Methods: Hydroalcoholic extract was prepared using a Soxhlet extractor and its antiproliferative activity
was studied by MTT assay. Then, the mRNA expressions of "large tumour suppressor kinases 1
and 2" (LATS1 and LATS2), "Yes1 Associated Transcriptional Regulator" (YAP1), and "Transcriptional
co‑activator with PDZ‑binding motif" (TAZ) were measured using real-time PCR.
Results: According to MTT, the viability was decreased significantly after 24 h treatment with A. wilhelmsii
at the concentrations of 800-1000 μg/ml and after 48 h treatment at the concentration of 400-
1000 μg/ml. While the mRNA levels of LATS1, TAZ, and YAP1 decreased significantly compared to
untreated cells at the concentration of 200 μg/ml after 48 h treatment. However, the mRNA expression
of LATS2 did not change.
Conclusion: Our findings showed that hydroalcoholic extract of A. wilhelmsii inhibited the viability of
lung cancer cells as well as it could decrease the expression of both oncogenes in the Hippo pathway.
However, it had suppressing effects on LATS1, which should be considered in further studies.
