Generic placeholder image

Current Chemical Biology

Editor-in-Chief

ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

Research Article

Hydroalcoholic Extract of Achillea Wilhelmsii Decreases the Expressions of Hippo Signaling Pathway-Associated Oncogenes in the A549 Lung Cancer Cell Line

Author(s): Fariba Nabatchian, Maryam Davoudi, Mojtaba Ashtiani*, Negin Davoudi and Reza Afrisham

Volume 17, Issue 2, 2023

Published on: 01 March, 2023

Page: [140 - 146] Pages: 7

DOI: 10.2174/2212796817666230214100146

Price: $65

Open Access Journals Promotions 2
Abstract

Background: Achillea wilhelmsii used in traditional Iranian medicine to treat a variety of disorders, has been proven to contribute to some signaling pathways in cancers. Evidence suggests that the Hippo pathway, which regulates organ size, is altered in a few conditions like lung cancer. In this regard, this study aimed to evaluate the effect of the hydroalcoholic extract of this plant on the viability and mRNA expression of some Hippo signaling pathway-associated oncogenes and suppressors in A549 lung cancer cell lines.

Methods: Hydroalcoholic extract was prepared using a Soxhlet extractor and its antiproliferative activity was studied by MTT assay. Then, the mRNA expressions of "large tumour suppressor kinases 1 and 2" (LATS1 and LATS2), "Yes1 Associated Transcriptional Regulator" (YAP1), and "Transcriptional co‑activator with PDZ‑binding motif" (TAZ) were measured using real-time PCR.

Results: According to MTT, the viability was decreased significantly after 24 h treatment with A. wilhelmsii at the concentrations of 800-1000 μg/ml and after 48 h treatment at the concentration of 400- 1000 μg/ml. While the mRNA levels of LATS1, TAZ, and YAP1 decreased significantly compared to untreated cells at the concentration of 200 μg/ml after 48 h treatment. However, the mRNA expression of LATS2 did not change.

Conclusion: Our findings showed that hydroalcoholic extract of A. wilhelmsii inhibited the viability of lung cancer cells as well as it could decrease the expression of both oncogenes in the Hippo pathway. However, it had suppressing effects on LATS1, which should be considered in further studies.

Keywords: Lung cancer, Achillea wilhelmsii, hippo, pathway, A549, hydroalcoholic extract.

Graphical Abstract
[1]
Pyenson, B.S.; Sander, M.S.; Jiang, Y.; Kahn, H.; Mulshine, J.L. An actuarial analysis shows that offering lung cancer screening as an insurance benefit would save lives at relatively low cost. Health Aff., 2012, 31(4), 770-779.
[http://dx.doi.org/10.1377/hlthaff.2011.0814] [PMID: 22492894]
[2]
Mousavi, S.M.; Gouya, M.M.; Ramazani, R.; Davanlou, M.; Hajsadeghi, N.; Seddighi, Z. Cancer incidence and mortality in Iran. Ann. Oncol., 2009, 20(3), 556-563.
[http://dx.doi.org/10.1093/annonc/mdn642] [PMID: 19073863]
[3]
Wu, Y.; Huang, Z.; Wang, S.; Yang, X.; Ou, W. A randomized trial of systematic nodal dissection in resectable non-small cell lung cancer. Lung Cancer, 2002, 36(1), 1-6.
[http://dx.doi.org/10.1016/S0169-5002(01)00445-7] [PMID: 11891025]
[4]
Collins, L.G.; Haines, C.; Perkel, R.; Enck, R.E. Lung cancer: diagnosis and management. Am. Fam. Physician, 2007, 75(1), 56-63.
[PMID: 17225705]
[5]
Jemal, A.; Siegel, R.; Ward, E.; Hao, Y.; Xu, J.; Murray, T.; Thun, M.J. Cancer Statistics, 2008. CA Cancer J. Clin., 2008, 58(2), 71-96.
[http://dx.doi.org/10.3322/CA.2007.0010] [PMID: 18287387]
[6]
Proctor, R.N. The history of the discovery of the cigarette–lung cancer link: evidentiary traditions, corporate denial, global toll: Table 1. Tob. Control, 2012, 21(2), 87-91.
[http://dx.doi.org/10.1136/tobaccocontrol-2011-050338] [PMID: 22345227]
[7]
Jordan, E.J.; Kim, H.R.; Arcila, M.E.; Barron, D.; Chakravarty, D.; Gao, J.; Chang, M.T.; Ni, A.; Kundra, R.; Jonsson, P.; Jayakumaran, G.; Gao, S.P.; Johnsen, H.C.; Hanrahan, A.J.; Zehir, A.; Rekhtman, N.; Ginsberg, M.S.; Li, B.T.; Yu, H.A.; Paik, P.K.; Drilon, A.; Hellmann, M.D.; Reales, D.N.; Benayed, R.; Rusch, V.W.; Kris, M.G.; Chaft, J.E.; Baselga, J.; Taylor, B.S.; Schultz, N.; Rudin, C.M.; Hyman, D.M.; Berger, M.F.; Solit, D.B.; Ladanyi, M.; Riely, G.J. Prospective comprehensive molecular characterization of lung adenocarcinomas for efficient patient matching to approved and emerging therapies. Cancer Discov., 2017, 7(6), 596-609.
[http://dx.doi.org/10.1158/2159-8290.CD-16-1337] [PMID: 28336552]
[8]
Ettinger, D.S.; Akerley, W.; Bepler, G.; Blum, M.G.; Chang, A.; Cheney, R.T. Non-small cell lung cancer: clinical practice guidelines in oncology. JNCCN J. Natl. Compr. Cancer Netw., 2010, 8, 740-741.
[http://dx.doi.org/10.6004/jnccn.2010.0056]
[9]
Dudley, J.T.; Karczewski, K.J. Exploring personal genomics; Oxford University Press, 2013.
[http://dx.doi.org/10.1093/acprof:oso/9780199644483.001.0001]
[10]
Lam, W.K. Lung cancer in Asian women-the environment and genes. Respirology, 2005, 10(4), 408-417.
[http://dx.doi.org/10.1111/j.1440-1843.2005.00723.x] [PMID: 16135162]
[11]
Ahn, D.H.; Gerber, D.E. Supportive Care in Lung Cancer. Lung Cancer, 2013, 201.
[PMID: 23910909]
[12]
Giard, D.J.; Aaronson, S.A.; Todaro, G.J.; Arnstein, P.; Kersey, J.H.; Dosik, H.; Parks, W.P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst., 1973, 51(5), 1417-1423.
[http://dx.doi.org/10.1093/jnci/51.5.1417] [PMID: 4357758]
[13]
Kang, Y.J.; Feng, Y.; Hatcher, E.L. Glutathione stimulates A549 cell proliferation in glutamine-deficient culture: The effect of glutamate supplementation. J. Cell. Physiol., 1994, 161(3), 589-596.
[http://dx.doi.org/10.1002/jcp.1041610323] [PMID: 7962140]
[14]
Bonfoco, E.; Krainc, D.; Ankarcrona, M.; Nicotera, P.; Lipton, S.A. Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc. Natl. Acad. Sci., 1995, 92(16), 7162-7166.
[http://dx.doi.org/10.1073/pnas.92.16.7162] [PMID: 7638161]
[15]
Heallen, T; Zhang, M; Wang, J; Bonilla-Claudio, M; Klysik, E Johnson, RL Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size. Science (80-) 2011, 332, 458-61.
[http://dx.doi.org/10.1126/science.1199010]
[16]
Harvey, K.F.; Zhang, X.; Thomas, D.M. The Hippo pathway and human cancer. Nat. Rev. Cancer, 2013, 13(4), 246-257.
[http://dx.doi.org/10.1038/nrc3458] [PMID: 23467301]
[17]
Baig, B.; Halim, S.A.; Farrukh, A.; Greish, Y.; Amin, A. Current status of nanomaterial-based treatment for hepatocellular carcinoma. Biomed. Pharmacother., 2019, 116108852
[http://dx.doi.org/10.1016/j.biopha.2019.108852] [PMID: 30999152]
[18]
Amin, A.; Mahmoud-Ghoneim, D. Texture analysis of liver fibrosis microscopic images: a study on the effect of biomarkers. Acta Biochim. Biophys. Sin., 2011, 43(3), 193-203.
[http://dx.doi.org/10.1093/abbs/gmq129] [PMID: 21258076]
[19]
El-Kharrag, R.; Amin, A.; Hisaindee, S.; Greish, Y.; Karam, S.M. Development of a therapeutic model of precancerous liver using crocin-coated magnetite nanoparticles. Int. J. Oncol., 2017, 50(1), 212-222.
[http://dx.doi.org/10.3892/ijo.2016.3769] [PMID: 27878253]
[20]
Al Shamsi, M.S.; Amin, A.; Adeghate, E. Beneficial effect of vitamin E on the metabolic parameters of diabetic rats. Mol. Cell. Biochem., 2004, 261(1), 35-42.
[http://dx.doi.org/10.1023/B:MCBI.0000028735.79172.9b] [PMID: 15362483]
[21]
Amin, A.; Farrukh, A.; Murali, C.; Soleimani, A.; Praz, F.; Graziani, G.; Brim, H.; Ashktorab, H. Saffron and its major ingredients’ effect on colon cancer cells with mismatch repair deficiency and microsatellite instability. Molecules, 2021, 26(13), 3855.
[http://dx.doi.org/10.3390/molecules26133855] [PMID: 34202689]
[22]
Mu, C.; Sheng, Y.; Wang, Q.; Amin, A.; Li, X.; Xie, Y. Potential compound from herbal food of Rhizoma Polygonati for treatment of COVID-19 analyzed by network pharmacology: Viral and cancer signaling mechanisms. J. Funct. Foods, 2021, 77104149
[http://dx.doi.org/10.1016/j.jff.2020.104149] [PMID: 32837538]
[23]
Ashtiani, M.; Nabatchian, F.; Galavi, H.R.; Saravani, R.; Farajian-Mashhadi, F.; Salimi, S. Effect of Achillea wilhelmsii extract on expression of the human telomerase reverse transcriptase mRNA in the PC3 prostate cancer cell line. Biomed. Rep., 2017, 7(3), 251-256.
[http://dx.doi.org/10.3892/br.2017.956] [PMID: 28811896]
[24]
Asgary, S.; Naderi, G.H.; Sarrafzadegan, N.; Mohammadifard, N.; Mostafavi, S.; Vakili, R. Antihypertensive and antihyperlipidemic effects of Achillea wilhelmsii. Drugs Exp. Clin. Res., 2000, 26(3), 89-93.
[PMID: 10941601]
[25]
Galavi, H.R.; Saravani, R.; Shahraki, A.; Ashtiani, M. Anti-proliferative and apoptosis inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract on human breast adenocarcinoma cell lines MCF-7 and MDA-Mb-468. Pak. J. Pharm. Sci., 2016, 29(6)(Suppl.), 2397-2403.
[PMID: 28167484]
[26]
Konsavage, W.M., Jr; Kyler, S.L.; Rennoll, S.A.; Jin, G.; Yochum, G.S. Wnt/β-catenin signaling regulates Yes-associated protein (YAP) gene expression in colorectal carcinoma cells. J. Biol. Chem., 2012, 287(15), 11730-11739.
[http://dx.doi.org/10.1074/jbc.M111.327767] [PMID: 22337891]
[27]
Ma, L.; Vaz, F.M.; Gu, Z.; Wanders, R.J.A.; Greenberg, M.L. The human TAZ gene complements mitochondrial dysfunction in the yeast taz1Δ; mutant. Implications for Barth syndrome. J. Biol. Chem., 2004, 279(43), 44394-44399.
[http://dx.doi.org/10.1074/jbc.M405479200] [PMID: 15304507]
[28]
Xia, H.; Qi, H.; Li, Y.; Pei, J.; Barton, J.; Blackstad, M.; Xu, T.; Tao, W. LATS1 tumor suppressor regulates G2/M transition and apoptosis. Oncogene, 2002, 21(8), 1233-1241.
[http://dx.doi.org/10.1038/sj.onc.1205174] [PMID: 11850843]
[29]
Stražišar, M.; Mlakar, V.; Glavač D. LATS2 tumour specific mutations and down-regulation of the gene in non-small cell carcinoma. Lung Cancer, 2009, 64(3), 257-262.
[http://dx.doi.org/10.1016/j.lungcan.2008.09.011] [PMID: 19008013]
[30]
Zygulska, A.L.; Krzemieniecki, K.; Pierzchalski, P. Hippo pathway - brief overview of its relevance in cancer. J. Physiol. Pharmacol., 2017, 68(3), 311-335.
[PMID: 28820389]
[31]
Carmichael, J.; DeGraff, W.G.; Gazdar, A.F.; Minna, J.D.; Mitchell, J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res., 1987, 47(4), 936-942.
[PMID: 3802100]
[32]
Hayon, T.; Dvilansky, A.; Shpilberg, O.; Nathan, I. Appraisal of the MTT-based assay as a useful tool for predicting drug chemosensitivity in leukemia. Leuk. Lymphoma, 2003, 44(11), 1957-1962.
[http://dx.doi.org/10.1080/1042819031000116607] [PMID: 14738150]
[33]
Van Peer, G.; Mestdagh, P.; Vandesompele, J. Accurate RT-qPCR gene expression analysis on cell culture lysates. Sci. Rep., 2012, 2(1), 222.
[http://dx.doi.org/10.1038/srep00222] [PMID: 22355736]
[34]
Schmittgen, T.D.; Livak, K.J. Analyzing real-time PCR data by the comparative CT method. Nat. Protoc., 2008, 3(6), 1101-1108.
[http://dx.doi.org/10.1038/nprot.2008.73] [PMID: 18546601]
[35]
Abdalla, Y.; Abdalla, A.; Hamza, A.A.; Amin, A. Safranal prevents liver cancer through inhibiting oxidative stress and alleviating inflammation. Front. Pharmacol., 2022 Feb 1; 12: 777500. eCollection 2021.
[http://dx.doi.org/10.3389/fphar.2021.777500] [PMID: 35177980]
[36]
Juaid, N.; Amin, A.; Abdalla, A.; Reese, K.; Alamri, Z.; Moulay, M.; Abdu, S.; Miled, N. Anti-hepatocellular carcinoma biomolecules: molecular targets insights. Int. J. Mol. Sci., 2021, 22(19), 10774.
[http://dx.doi.org/10.3390/ijms221910774] [PMID: 34639131]
[37]
Hamza, A.A.; Heeba, G.H.; Hamza, S.; Abdalla, A.; Amin, A. Standardized extract of ginger ameliorates liver cancer by reducing proliferation and inducing apoptosis through inhibition oxidative stress/inflammation pathway. Biomed. Pharmacother., 2021, 134111102
[http://dx.doi.org/10.1016/j.biopha.2020.111102] [PMID: 33338743]
[38]
Xu, Z.P.; Zhu, J.S.; Zhang, Q.; Wang, X.Y. A breakdown of the Hippo pathway in gastric cancer. Hepatogastroenterology, 2011, 58(110-111), 1611-1617.
[http://dx.doi.org/10.5754/hge10669] [PMID: 21940329]
[39]
Plouffe, S.W.; Hong, A.W.; Guan, K.L. Disease implications of the Hippo/YAP pathway. Trends Mol. Med., 2015, 21(4), 212-222.
[http://dx.doi.org/10.1016/j.molmed.2015.01.003] [PMID: 25702974]
[40]
Muramatsu, T.; Imoto, I.; Matsui, T.; Kozaki, K.; Haruki, S.; Sudol, M.; Shimada, Y.; Tsuda, H.; Kawano, T.; Inazawa, J. YAP is a candidate oncogene for esophageal squamous cell carcinoma. Carcinogenesis, 2011, 32(3), 389-398.
[http://dx.doi.org/10.1093/carcin/bgq254] [PMID: 21112960]
[41]
Zhou, Z.; Hao, Y.; Liu, N.; Raptis, L.; Tsao, M.S.; Yang, X. TAZ is a novel oncogene in non-small cell lung cancer. Oncogene, 2011, 30(18), 2181-2186.
[http://dx.doi.org/10.1038/onc.2010.606] [PMID: 21258416]
[42]
Cordenonsi, M.; Zanconato, F.; Azzolin, L.; Forcato, M.; Rosato, A.; Frasson, C.; Inui, M.; Montagner, M.; Parenti, A.R.; Poletti, A.; Daidone, M.G.; Dupont, S.; Basso, G.; Bicciato, S.; Piccolo, S. The Hippo transducer TAZ confers cancer stem cell-related traits on breast cancer cells. Cell, 2011, 147(4), 759-772.
[http://dx.doi.org/10.1016/j.cell.2011.09.048] [PMID: 22078877]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy