![conference banner](/images/conference-banner.jpeg)
Abstract
The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.
Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome
Current Pharmaceutical Biotechnology
Title: Long-Short-Long Games in mRNA Identification: The Length Matters
Volume: 9 Issue: 5
Author(s): San Ming Wang
Affiliation:
Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome
Abstract: The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.
Export Options
About this article
Cite this article as:
Wang Ming San, Long-Short-Long Games in mRNA Identification: The Length Matters, Current Pharmaceutical Biotechnology 2008; 9 (5) . https://dx.doi.org/10.2174/138920108785915166
DOI https://dx.doi.org/10.2174/138920108785915166 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
Latest Advancements in Biotherapeutics
The scope of this thematic issue is to comprehensively explore the rapidly evolving landscape of biotherapeutics, emphasizing breakthroughs in precision medicine. Encompassing diverse therapeutic modalities, the issue will delve into the latest developments in monoclonal antibodies, CRISPR/Cas gene editing, CAR-T cell therapies, and innovative drug delivery systems, such as nanoparticle-based ...read more
![Wayfinder Image](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers