![conference banner](/images/conference-banner.jpeg)
Abstract
Since Serial Analysis of Gene Expression (SAGE) was introduced more than a decade ago, it has been widely applied to characterise gene expression profiles in various tissues, cell types and cell lines of diverse origin including human, mouse, rat, yeast, plant and parasites. Throughout the past years many modifications to the original SAGE protocol have been developed, which address several aspects of SAGE, including an increase in sequencing efficiency (deep- SAGE), improved tag-to-transcript mapping of SAGE tags (LongSAGE) and a reduction of the amount of required input RNA (microSAGE). Furthermore, the applications of SAGE have expanded from exclusively transcriptome analysis to now also include genome analysis, identifying genome signature tags that pinpoint transcription factor binding sites throughout the genome (Serial Analysis of Chromatin Occupancy or SACO). The review gives an overview of the main modifications to the SAGE technology that have been developed in the last decade, with a particular focus on the large reduction in the amount of required input RNA that has been achieved in the many SAGE modifications for downscaling or miniaturisation of SAGE (including microSAGE, PCR-SAGE and small amplified RNA-SAGE). The available methods for downscaling or miniaturisation of SAGE and their specific features will be discussed, illustrated by some examples of their application. This reduction in required quantity of input RNA has greatly expanded the possible applications of SAGE, allowing characterisation of global gene expression in material obtained from needle biopsies, small anatomical structures and specific cell types isolated by fluorescence activated cell sorting or laser microdissection.
Keywords: SAGE, gene expression, downscaling, laser microdissection, microSAGE
Current Pharmaceutical Biotechnology
Title: Scaling Down SAGE: from miniSAGE to microSAGE
Volume: 9 Issue: 5
Author(s): N. A. Datson
Affiliation:
Keywords: SAGE, gene expression, downscaling, laser microdissection, microSAGE
Abstract: Since Serial Analysis of Gene Expression (SAGE) was introduced more than a decade ago, it has been widely applied to characterise gene expression profiles in various tissues, cell types and cell lines of diverse origin including human, mouse, rat, yeast, plant and parasites. Throughout the past years many modifications to the original SAGE protocol have been developed, which address several aspects of SAGE, including an increase in sequencing efficiency (deep- SAGE), improved tag-to-transcript mapping of SAGE tags (LongSAGE) and a reduction of the amount of required input RNA (microSAGE). Furthermore, the applications of SAGE have expanded from exclusively transcriptome analysis to now also include genome analysis, identifying genome signature tags that pinpoint transcription factor binding sites throughout the genome (Serial Analysis of Chromatin Occupancy or SACO). The review gives an overview of the main modifications to the SAGE technology that have been developed in the last decade, with a particular focus on the large reduction in the amount of required input RNA that has been achieved in the many SAGE modifications for downscaling or miniaturisation of SAGE (including microSAGE, PCR-SAGE and small amplified RNA-SAGE). The available methods for downscaling or miniaturisation of SAGE and their specific features will be discussed, illustrated by some examples of their application. This reduction in required quantity of input RNA has greatly expanded the possible applications of SAGE, allowing characterisation of global gene expression in material obtained from needle biopsies, small anatomical structures and specific cell types isolated by fluorescence activated cell sorting or laser microdissection.
Export Options
About this article
Cite this article as:
Datson A. N., Scaling Down SAGE: from miniSAGE to microSAGE, Current Pharmaceutical Biotechnology 2008; 9 (5) . https://dx.doi.org/10.2174/138920108785915120
DOI https://dx.doi.org/10.2174/138920108785915120 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
Latest Advancements in Biotherapeutics
The scope of this thematic issue is to comprehensively explore the rapidly evolving landscape of biotherapeutics, emphasizing breakthroughs in precision medicine. Encompassing diverse therapeutic modalities, the issue will delve into the latest developments in monoclonal antibodies, CRISPR/Cas gene editing, CAR-T cell therapies, and innovative drug delivery systems, such as nanoparticle-based ...read more
![Wayfinder Image](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Shift in the “Paradigm” of the Pharmacology of Hypertension
Current Topics in Medicinal Chemistry Optical Imaging of Microvascular Morphology and Perfusion
Current Angiogenesis (Discontinued) Secondary Prevention of Ischemic Stroke
Current Drug Targets Smoking and Cardiovascular System: Cellular Features of the Damage
Current Pharmaceutical Design Kinin Receptors in Vascular Biology and Pathology
Current Vascular Pharmacology <i>Uncaria rhynchophylla</i> and its Major Constituents on Central Nervous System: A Review on Their Pharmacological Actions
Current Vascular Pharmacology Preeclampsia: Placental Origins, New Predictors and New Therapeutic Strategies
Current Women`s Health Reviews Therapeutic Implications of Tocilizumab, A Humanized Anti-Interleukin-6 Receptor Antibody, for Various Immune-Mediated Diseases: An Update Review
Current Rheumatology Reviews Editorial [Hot topic: Modifying Cardiovascular Risk Factors: Epidemiology and Characteristics of Smoking-Related Cardiovascular Diseases (Executive Editor: Aurelio Leone)]
Current Pharmaceutical Design Value of Real Time Three Dimensional Transesophageal Echocardiography in General Cardiology Practice
Recent Patents on Medical Imaging Toxics of Tobacco Smoke and Cardiovascular System: From Functional to Cellular Damage
Current Pharmaceutical Design Vascular Stents in the Management of Intracranial Aneurysms
Recent Patents on Medical Imaging The Role of Endothelial Dysfunction in Aortic Aneurysms
Current Pharmaceutical Design The Injured Cochlea as a Target for Inflammatory Processes, Initiation of Cell Death Pathways and Application of Related Otoprotective Strategies
Recent Patents on CNS Drug Discovery (Discontinued) Thrombolytic Therapy in Peripheral Arterial Disease
Current Drug Targets - Cardiovascular & Hematological Disorders Adrenergic and V1-ergic Agonists/Antagonists Affecting Recovery from Brain Trauma in the Lund Project Act on Astrocytes
Current Signal Transduction Therapy Currently Used Biologic Agents in the Management of Behcet’s Syndrome
Current Medicinal Chemistry Diabetic Papillopathy: Current and New Treatment Options
Current Diabetes Reviews Nimodipine Loaded PLGA Nanoparticles: Formulation Optimization Using Factorial Design,Characterization and In Vitro Evaluation
Current Drug Delivery Beyond Lipoprotein Receptors: Learning from Receptor Knockouts Mouse Models about New Targets for Reduction of the Atherosclerotic Plaque.
Current Molecular Medicine