Title:Amino Acids Profile in Children with Acute Brucellosis
Volume: 20
Issue: 2
Author(s): Ahmet Guzelcicek, Nihayet Bayraktar and Mehmet Bayraktar*
Affiliation:
- Department of Medical Microbiology, Harran University, Medical School, Sanliurfa, Turkey
Keywords:
Amino acids profiles, acute brucellosis, pediatrics population, JASEM amino acid kit, LC-MS device, unpasteurized cheese.
Abstract:
Background: Many new cases of brucella infections are seen in Turkey every year, especially
in March, April, and May, due to the consumption of local unpasteurized cheese. Amino acids
profiles have not been studied in brucellosis so far.
Aims: The amino acid profiles may be affected by infectious diseases. Our study aims to evaluate
the plasma amino acid profile in the progression of acute brucellosis.
Methods: Plasma amino acid profile was performed by an 8045 LC-MS / MS device (Shimadzu
8045, Japan) using JASEM amino acid kit.
Results: Analysis of 45 amino acid profiles was made and results profiles showed significant differences
in concentrations and types of amino acids in brucella patients. We observed a significant difference
in terms of alanine, arginine, aspartic acid, glutamine, glutamic acid, glycine, isoleucine,
ornithine, phenylalanine, proline, tyrosine, valine, alpha-aminoadipic acid, alpha-amino-pimelic acid,
argininosuccinic acid, gamma-aminobutyric acid, thiaproline, 1-methylhistidine, 3-methylhistidine,
hydroxylysine, hydroxyproline, cystine, serotonin, ethanolamine, and taurine (p-value < 0.05
for each). No significant differences were determined regarding asparagine, citrulline, histidine,
leucine, alloisoleucine, lysine, methionine, serine, threonine, tryptophan, anserine, alpha aminobutyric
acid, beta aminoisobutyric acid, beta-alanine, cystathionine, histamine, and 5-oh-trp (p-value >
0.05 for all).
Conclusion: Patients with brucellosis have a specific profile of amino acids which may reflect sequelae
of pathological and metabolic biochemical changes in the disease process due to the growth
of Brucella spp. in the human body leading to an imbalance of amino acid levels.