Title:Microfluidic Paper-based Device for Medicinal Diagnosis
Volume: 22
Issue: 27
Author(s): Atchara Lomae, Pattarachaya Preechakasedkit, Kanyapat Teekayupak, Yosita Panraksa, Jutiporn Yukird, Orawon Chailapakul*Nipapan Ruecha*
Affiliation:
- Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science,
Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand
- Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science,
Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand
- Metallurgy and Materials
Science Research Institute, Chulalongkorn University, Soi Chula 12, Phayathai Rd., Pathumwan, Bangkok, 10330,
Thailand
Keywords:
Paper-based analytical device, Microfluidic paper-based analytical devices (μPADs), Medicinal diagnosis, Pointof- care testing (POC), Device design, Lateral flow assay (LFA), Two-dimensional (2D) configurations, Three-dimensional (3D) configuration.
Abstract:
Background: The demand for point-of-care testing (POCT) devices has rapidly grown
since they offer immediate test results with ease of use, makingthem suitable for home self-testing
patients and caretakers. However, the POCT development has faced the challenges of increased cost
and limited resources. Therefore, the paper substrate as a low-cost material has been employed to
develop a cost-effective POCT device, known as “Microfluidic paper-based analytical devices
(μPADs)”. This device is gaining attention as a promising tool for medicinal diagnostic applications
owing to its unique features of simple fabrication, low cost, enabling manipulation flow (capillarydriven
flow), the ability to store reagents, and accommodating multistep assay requirements.
Objective: This review comprehensively examines the fabrication methods and device designs
(2D/3D configuration) and their advantages and disadvantages, focusing on updated μPADs applications
for motif identification.
Methods: The evolution of paper-based devices, starting from the traditional devices of dipstick and
lateral flow assay (LFA) with μPADs, has been described. Patterned structure fabrication of each
technique has been compared among the equipment used, benefits, and drawbacks. Microfluidic device
designs, including 2D and 3D configurations, have been introduced as well as their modifications.
Various designs of μPADs have been integrated with many powerful detection methods such
as colorimetry, electrochemistry, fluorescence, chemiluminescence, electrochemiluminescence, and
SER-based sensors for medicinal diagnosis applications.
Conclusion: The μPADs potential to deal with commercialization in terms of the state-of-the-art of
μPADs in medicinal diagnosis has been discussed. A great prototype, which is currently in a reallife
application breakthrough, has been updated.
