Title:Evaluation of the Antioxidant, Antidiabetic, and Anticholinesterase Potential
of Biogenic Silver Nanoparticles from Khaya grandifoliola
Volume: 11
Issue: 1
Author(s): Jude Akinyelu*, Abiodun Aladetuyi, Londiwe Simphiwe Mbatha and Olakunle Oladimeji
Affiliation:
- Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria
Keywords:
Nanoparticles, biogenic, antioxidant, antidiabetic, anticholinesterase, reactive oxygen species.
Abstract:
Introduction: In recent years, plant-mediated synthesis of silver nanoparticles has
evolved as a promising alternative to traditional synthesis methods. In addition to producing silver
nanoparticles with diverse biomedical potential, the biosynthesis approach is known to be inexpensive,
rapid, and environmentally friendly.
Objective: This study was aimed at synthesizing silver nanoparticles using ethanolic stem and root
bark extracts of Khaya grandifoliola and highlighting the biomedical potential of the nanoparticles
by evaluating their antioxidant, antidiabetic and anticholinesterase effects in vitro.
Methods: Silver nanoparticles were prepared using ethanolic stem and root bark extracts of K.
grandifoliola as precursors. The biogenic silver nanoparticles were characterized using UV-visible
spectroscopy, fourier transform infrared spectroscopy, scanning electron microscopy and energydispersive
X-ray analysis. Furthermore, 2,2-Diphenyl-1-picrylhydrazyl radical scavenging, ferric
ion reducing antioxidant power, and nitric oxide scavenging assays were used to determine the
antioxidant property of the nanoparticles. The antidiabetic potential of the nanoparticles was determined
by evaluating their inhibitory effect on the activity of α-amylase and α-glucosidase. The
anticholinesterase potential of the nanoparticles was determined by assessing their inhibitory effect
on the activity of acetylcholinesterase and butyrylcholinesterase.
Results: UV-visible spectroscopy showed surface plasmon resonance bands between 425 and 450
nm. Scanning electron microscopy revealed almost round nanoparticles with a maximum size of
91 nm. Fourier transform infrared spectroscopy affirmed the role of the phytoconstituents present
in K. grandifoliola as reducing and stabilizing agents. The biogenic silver nanoparticles showed
remarkable antioxidant, antidiabetic, and anticholinesterase effects.
Conclusion: Biogenic silver nanoparticles could be useful in biomedical and pharmacological
applications.