Title:Evaluation and Application of ZIC-HILIC Columns Selectivity for Four
Angiotensin II Receptor Blockers in Pharmaceutical Formulations
Volume: 18
Issue: 9
Author(s): Ayad K. Fadhil, Ashraf S. Rasheed*Mohammed Jasim M. Hassan
Affiliation:
- Department of Chemistry, College of Science,
University of Baghdad, Al-Jadriya Campus, 10071 Baghdad, Iraq
Keywords:
Hypertension, angiotensin II, receptor blockers, HILIC, pharmaceutical, formulations.
Abstract:
Background: Angiotensin II receptor blockers (ARBs) are helpful medications for
treating hypertension and heart failure and have been proposed as a possible alternative to standard
angiotensin-converting enzyme inhibitors.
Objective: The present article describes and validates sensitive hydrophilic interaction liquid
chromatography methods to simultaneously analyse four angiotensin II receptor blockers: valsartan,
telmisartan, losartan, and irbesartan.
Methods: Numerous chromatographic parameters were studied and optimized in detail. As a mobile
phase, the best separation was obtained on two handmade columns (ZIC-S1 and ZIC-S4) using
acetonitrile/sodium acetate buffer (40 mM, pH 4.75). The detection of target pharmaceuticals
was obtained at 220 nm.
Results: The linear ranges of target drugs (valsartan, telmisartan, losartan, and irbesartan) were
0.02-4, 0.06-7, 0.005-6 and 0.08-5 μg/mL on the ZIC-S1 and ZIC-S4 columns, respectively. The
suggested methods demonstrated high precision (RSD ≤ 1.15), linearity (r2 ≥ 0.9964), and accuracy
(between 98.88 and 101%) with detection and quantitation limits of (0.0021-0.0550 and
0.0063-0.1666 μg/mL, respectively) for all target pharmaceuticals.
Conclusion: The suggested methods have been validated and applied to pharmaceutical formulations,
indicating that it is suitable for therapeutic drug monitoring of these medications.
