Title:Long Non-coding RNA UCA1 Regulates SRPK1 Expression Through miR-
99b-3p in Ovarian Cancer
Volume: 29
Issue: 10
Author(s): Juan Xu, Liu-hong Zheng, Yi-nuo Hong, Cheng Xuan, Shu-ling Yan, Guo-Liang Lv, Zheng-Gang Jiang*Xian-Feng Ding*
Affiliation:
- Department of Science Research and Information
and Management, Zhejiang Provincial Centers for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
- College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
Keywords:
lncRNA UCA1, miR-99b-3p, ovarian cancer, SRPK1, gynecological malignancy, UTR.
Abstract:
Background: Ovarian carcinoma (OC) is one of the most common malignancies of the
female reproductive organs, with a low survival rate primarily due to the lack of effective methods
for early diagnosis and prognosis.
Objective: In this article, our motivation is to explore the lncRNA-related network mechanisms
involved in the pathogenesis of OC.
Methods: Public lncRNAs and mRNA expression datasets for OC were collected from the Gene
Expression Omnibus (GEO) database. By integrated bioinformatics analysis, we constructed a
UCA1-miRNA-mRNA network. We studied lncRNA-related molecular modulation mechanism in
ovarian cancer cells based on MTT assay, dual luciferase reporter gene assays, quantitative realtime
PCR, and western blotting.
Results: UCA1 was higher in ovarian tumor tissues and cells than normal tissues and cells. It was
demonstrated in this study that knockdown of UCA1 inhibited ovarian cancer cell viability, which a
miR-99b-3p inhibitor could reverse in vitro. Further, UCA1 was shown to regulate the expression of
SRPK1 by directly binding to miR-99b-3p.
Conclusion: These results suggest that UCA1 functions as an oncogene in ovarian cancer.
Inhibition of UCA1/miR-99b-3p/SRPK1 axis may become a novel target for treating ovarian
cancer.
