Title:8-oxoguanine DNA Glycosylase (OGG1) may be a Diagnostic Indicator of
Diminished Ovarian Reserve (DOR)
Volume: 26
Issue: 5
Author(s): Jing Xia, Zehao Wang, Wen Zou, Wenyi Jin, Gengxiang Wu*Jing Yang*
Affiliation:
- Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan 430060, China
- Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan 430060, China
Keywords:
Diminished ovarian reserve, oxidative stress, 8-oxoguanine DNA glycosylase, anti-Müllerian hormone, folliclestimulating hormone, diagnostic.
Abstract:
Background: Oxidative/antioxidant imbalance is considered a causal cause of diminished
ovarian reserve (DOR). 8-oxyguanine DNA glycosylase (OGG1) has been reported to act as an antioxidant
by binding non-catalytically to oxidation-induced DNA damage in the promoter region.
Objective: This study aimed to evaluate serum OGG1 concentrations in patients with or without
DOR and to explore the clinical value of OGG1 as a novel diagnostic indicator for DOR.
Methods: Sixty-four women with DOR and seventy-eight women with normal ovarian reserve
(NOR) from the reproductive medical center of Renmin Hospital of Wuhan University were included.
Enzyme-linked immunosorbent assay (ELISA) kits were used to determine serum OGG1
levels in patients on 2-5 days of the menstrual cycle. Data regarding the enrolled patients were also
obtained from the database of the hospital, including age, body mass index (BMI), anti-Müllerian
hormone (AMH), etc.
Results: OGG1 levels were increased in the DOR group (2.08 ± 0.70 vs 1.46 ± 0.47 nmol/L,
P < 0.001) and negatively correlated with AMH levels (Spearman r = -0.586, P < 0.001). After adjusting
for age and BMI, a negative association between OGG1 and AMH remained (β = -0.619, P
< 0.001). ROC curve analysis showed that a cut-off value of 1.765 nmol/L had an appropriate sensitivity
(81.30%) and specificity (76.90%) for discriminating individuals with and without DOR,
with the area under the curve (95% CI) of 0.870 (0.814 to 0.926), P < 0.001.
Conclusion: We determined that serum OGG1 levels might be suggested as a new diagnostic indicator
for DOR.
