Title:Electrochemiluminescence Sensor based on Electrospun Crosslinked
Carbon Nanofibers for the Detection of Difenidol Hydrochloride
Volume: 26
Issue: 6
Author(s): Hao Cheng, Xuenuan Li, Tianhao Li, Tingfan Tang and Danfeng Qin*
Affiliation:
- Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering,
Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, P.R. China
Keywords:
Genipin, crosslinking agent, CNF, electrochemiluminescence, Ru(bpy)3 2+, difenidol hydrochloride.
Abstract:
Background: Cross-linked porous carbon nanofibers (CNF) were successfully prepared
by electrospinning and high-temperature carbonization. Polyacrylonitrile (PAN) as the carbon
source and genipin as the cross-linking agent were used to prepare cross-linked porous carbon nanofibers
(CNF).
Materials and Methods: The field emission scanning electron microscopy (SEM), transmission
electron microscope (TEM), automatic specific surface and porosity analyzer Brunner Emmet
Teller (BET), X-ray diffraction (XRD), and a laser confocal microspectroscope (Raman, XploRA
PLUS, Horiba) were used to characterize the materials. The CNF suspension was dropped on the
surface of the bare glassy carbon electrode by the drip coating method to obtain a CNF-modified
electrode. Cyclic voltammetry was used to study the electrochemiluminescence behavior of difenidol
hydrochloride on CNF-modified glassy carbon electrode (Glassy Carbon Electrode, GCE).
Results and Discussion: Herein, we synthesised a kind of crosslinked carbon nanofibers and designed
a novel ECL biosensor. Under the optimal conditions, the concentration of difenidol hydrochloride
exhibited a linear relationship with the peak current in the range of 8.0×10-8 to 1.0×10-4
mol/L, with the correlation coefficient of R2=0.997, and a low detection limit (1.2×10-8 mol/L).
Difenidol hydrochloride in difenidol hydrochloride tablets was tested, and the recovery rate of
sample addition was estimated to be 83.17%-92.17%, and the RSD value to be <5.0%. The designed
platform exhibited excellent analytical performance for difenidol hydrochloride determination.