Title:Bone Marrow Mesenchymal Stem Cells Derived from Juvenile Macaques
Reversed the Serum Protein Expression Profile in Aged Macaques
Volume: 18
Issue: 3
Author(s): Qianqian Yu, Chuan Tian, Guanke Lv, Qingpeng Kong, Gonghua Li, Guangxu Zhu, Xiangqing Zhu*Xinghua Pan*
Affiliation:
- The Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions, Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming Key Laboratory of Stem Cell and Regenerative Medicine, Basic Medical Laboratory, 920th Hospital of Joint Logistics Support Force, PLA, Kunming, 650032 ,Yunnan Province, China
- The Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions, Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming Key Laboratory of Stem Cell and Regenerative Medicine, Basic Medical Laboratory, 920th Hospital of Joint Logistics Support Force, PLA, Kunming, 650032 ,Yunnan Province, China
Keywords:
Bone marrow mesenchymal stem cells, ageing, serum proteins, ageing-related indicators, tumours, juvenile macaques.
Abstract:
Objective: The aim of the study was to reveal the changes in serum protein composition and
content in macaques during the process of ageing, and explore the effect of bone marrow mesenchymal
stem cell (BMMSC) on the serum protein expression profile in elderly macaques.
Methods: Naturally ageing macaques were assessed according to age. BMMSCs were intravenously infused
into aged macaques. In addition, peripheral blood was collected to obtain serum for dataindependent
acquisition (DIA) protein sequencing to identify aging-related indicators. One hundred
eighty days after macaques received BMMSC treatment, haemoxylin and eosin (HE) staining was performed
to observe the morphology and structure of aortic arches.
Results: Compared to infant and young control macaques, aged macaques showed erythema on the face,
dry skin, reduced amounts of hair on the head and back, and paleness. Cultured BMMSCs from the 4th
passage (P4 BMMSCs) were grown in accordance with standards used to culture mesenchymal stem
cells. After BMMSC treatment, the assessed aortic arches showed no calcium salt deposition or cell necrosis,
and the characteristics of the serum protein expression profile tended to be similar to that of the
infant and young groups, with the expression of 41 proteins upregulated with age and that of 30 proteins
downregulated with age but upregulated after BMMSC treatment. Moreover, we identified 44 significantly
differentially expressed proteins between the aged model and treatment groups; 11 of the upregulated
proteins were related to vascular ageing, neuronal ageing and haematopoiesis, and 33 of the downregulated
proteins were associated with neuronal ageing, cardiovascular disease, and tumours. Interestingly,
S100 expression in serum was significantly decreased, COMP expression was significantly increased,
NKAP expression reappeared, and LCN2, CSF1R, CORO1C, CSTB and RSU-1 expression disappeared
after BMMSC treatment.
Conclusion: BMMSCs can reverse ageing-related serum protein expression.
