Title:Antioxidation Abilities of Sesquiterpenoids from Curcuma aromatica
in vitro
Volume: 26
Issue: 1
Author(s): Weifeng Dai, Dong Wang, Chonglian Chen and Mi Zhang*
Affiliation:
- Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Keywords:
Antioxidation, sesquiterpenoids, Curcuma aromatica, PLS analysis, bioactivity, PC12.
Abstract:
Aims and Objectives: In our previous study, 21 sesquiterpenoids with different skeleton
types were isolated from the radix of Curcuma aromatica Salisb., a traditional Chinese medicine
used for treating depression and qi and blood stasis. C. aromatica enhanced PC12 cell viability upon
damage by H2O2. The aim of this study was to elucidate the antioxidation capability of these
sesquiterpenoids using a model of H2O2-induced PC12 cells and analyze the correlation between
their structure and bio-activity.
Materials and Methods: PC12 cells were simultaneously treated with 400 μM H2O2 and sesquiterpenoid
compounds or vitamin E (used as a positive control) for 24 h. The activities of GSH-Px,
LDH, CAT, and SOD were detected by ELISA kits. The reactive oxygen species (ROS) level in
the cells was determined by the fluorescence probe DCFH-DA. [Ca2+]i was detected based on the
Fluo 2-AM fluorescence labeling assay. The structures of sesquiterpenoids were featured with 41
selected molecular descriptors, and the relationship between active parameters and structural features
was determined by the partial least squares (PLS) analysis.
Results: All twenty-one sesquiterpenoids from the radix of C. aromatica increased the activities of
GSH-Px, CAT, and SOD, and decreased the LDH leakage, and levels of ROS level and [Ca2+]i to
different degrees. Some relationships were observed between the molecular descriptors featured by
the sesquiterpenoids and GSH-Px, CAT, SOD, LDH, ROS, and [Ca2+]i by PLS analysis.
Conclusions: Twenty-one sesquiterpenoids showed different antioxidation abilities as measured by
a model of H2O2-induced PC12 cells. Five molecular descriptors were positively correlated with
GSH-Px, CAT, SOD, and were negatively correlated with LDH and [Ca2+].
