Title:Exploration of the Potential Mechanisms of Lingqihuangban Granule for
Treating Diabetic Retinopathy Based on Network Pharmacology
Volume: 26
Issue: 1
Author(s): Shuai He*, Chufeng Gu*, Tong Su*, Chuandi Zhou, Thashi Lhamo, Deji Draga, Lili Yin and Qinghua Qiu
Affiliation:
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine,
Shanghai, PR China
- National Clinical Research Center for Eye Diseases; Shanghai Key Laboratory of Ocular Fundus
Diseases; Shanghai Engineering Center for Visual Science and Photomedicine; Shanghai Engineering Center for Precise
Diagnosis and Treatment of Eye Diseases, Shanghai, PR China
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine,
Shanghai, PR China
- National Clinical Research Center for Eye Diseases; Shanghai Key Laboratory of Ocular Fundus
Diseases; Shanghai Engineering Center for Visual Science and Photomedicine; Shanghai Engineering Center for Precise
Diagnosis and Treatment of Eye Diseases, Shanghai, PR China
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine,
Shanghai, PR China
- National Clinical Research Center for Eye Diseases; Shanghai Key Laboratory of Ocular Fundus
Diseases; Shanghai Engineering Center for Visual Science and Photomedicine; Shanghai Engineering Center for Precise
Diagnosis and Treatment of Eye Diseases, Shanghai, PR China
Keywords:
Lingqihuangban granule, diabetic retinopathy, network pharmacology, Chinese herbal compound, retinal hemorrhage, photocoagulation.
Abstract:
Background: The Lingqihuangban Granule (LQHBG), a remarkable Chinese herbal
compound, has been used for decades to treat diabetic retinopathy (DR) in the Department of Ophthalmology,
Shanghai General Hospital (National Clinical Research Center for Eye Diseases) with
obvious effects. Through the method of network pharmacology, the present study constructed bioactive
component-relative targets and protein-protein interaction network of the LQHBG and implemented
gene function analysis and pathway enrichment of targets, discussing the mechanisms
of traditional Chinese medicine LQHBG in treating DR.
Materials and Methods: The bioactive ingredients of LQHBG were screened and obtained using
TCMSP and ETCM databases, while the potential targets of bioactive ingredients were predicted
by SwissTargetPrediction and ETCM databases. Compared with the disease target databases of
TTD, Drugbank, OMIM and DisGeNET, the therapeutic targets of LQHBG for DR were extracted.
Based on the DAVID platform, GO annotation and KEGG pathway analyses of key targets were
explored, combined with the screening of core pathways on the Omicshare database and pathway
annotation on the Reactome database.
Results: A total of 357 bioactive components were screened from LQHBG, involving 86 possible targets
of LQHBG treating DR. In the PPI network, INS and ALB were identified as key genes. The effective
targets were enriched in multiple signaling pathways, such as PI3K/Akt and MAPK pathways.
Conclusion: This study revealed the possible targets and pathways of LQHBG treating DR, reflecting
the characteristics of multicomponent, multitarget and multipathway treatment of a Chinese
herbal compound, and provided new ideas for further discussion.